Project Details
Projekt
Z-Project - Bacteriophage Repository, Services and Database
Applicant
Dr. Johannes Wittmann
Subject Area
Virology
Metabolism, Biochemistry and Genetics of Microorganisms
Metabolism, Biochemistry and Genetics of Microorganisms
Term
since 2021
Project identifier
Deutsche Forschungsgemeinschaft (DFG) - Project number 465136285
Hosted by the Leibniz Institute DSMZ and building on its expertise and previous work the Z project will provide a dedicated, tailored service portfolio across the three project areas (PAs) of the SPP, Viral Cell Biology (PA A), Unicellular and Multicellular Anti-viral Defenses (PA B), and Viral Impact on Multispecies Microbial Communities (PA C) for the participating research projects. This comprises support through the offer of state-of-the-art methodologies for all relevant aspects of the biology of prokaryotic viruses, the long-term conservation, quality control, and distribution of the isolated bacteriophages and prokaryotic hosts, as well as the associated data. The portfolio of the Z-project thus comprises: • isolation of bacteriophages against bacteria of interest, e.g. streptomycetes, Staphylococcus species or cyanobacteria; • isolation of target viruses and host bacteria/archaea as identified by metagenomic studies of complex microbial communities; • the sustainable deposit and provision of novel bacteriophages and their host strains in all PAs; • phage classification and nomenclatural support using an improved classification system, determination of the evolutionary related phage groups • determination of bacteriophage host ranges and lifestyle-relevant parameters, i.e. kinetics of phage replication and host cell lysis, latency time, burst size; • analysis of phage morphology by transmission electron microscopy; • long-read sequencing of phage genomes, and respective bioinformatic analyses; • maintaining and selective further development of PhageDive (PhageDive) that covers all relevant aspects of bacteriophage biology with specific data fields PhageDive employs controlled vocabulary and feature extensive search functions that will be programmed according to the specific needs of the SPP member projects. The current version will be regularly updated and more data from further research groups as well as data fields like for receptors or transcriptomic data will be implemented
DFG Programme
Priority Programmes
