Topogenesis and assembly of nucleolar proteins

• Antragstellerin: Privatdozentin Dr. Marion-Susanne Schmidt-Zachmann
• Fachliche Zuordnung: Zellbiologie
• Förderung: Förderung von 1998 bis 2007
• Projektkennung: Deutsche Forschungsgemeinschaft (DFG) - Projektnummer 5107068

Morphologically, the nucleolus - known to be the main site of ribosome biosynthesis - represents the most conspicuous structure in the eukaryotic cell nucleus. Recently, it has been demonstrated that the nucleolus may have additional functions in the assembly and/or transport of various ribonucleoprotein particles. The nucleolus is characterized by the presence of a specific set of ptoeins, which accumulate in distinct subnuclolar compartaments. In continuance of our previous work and encouraged by our recent results, we want to identify novel non-ribosomal nucleolar proteins. The use of immunological, biochemical and molecularbiological methods should allow a detailed characterization of these proteins as well as the determination of their specific intranucleolar localization. Moreover, the analysis and identification of interacting components within the nucleolus, be it on the nucleic acid- or protein level, might point to their biological functions. A major focus of these studies will be the identification of proteins which are involved in maintaining or even contributing to the nucleolar structural integrity independent of ongoing transcription, i.e. proteins of the so-called 'nucleolar skeleton'.

• DFG-Verfahren: Schwerpunktprogramme
• Teilprojekt zu: SPP 1050: Funktionelle Architektur des Zellkerns