C03 Mass spectrometry of RNA and proteins in modified RNPs (C03)

• Subject Area: Biochemistry
• Term: since 2021
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 439669440

Mass spectrometry has become a powerful tool to study protein-RNA interactions. However, as standard analyses remain typically limited to post-translational protein modifications, very few of these approaches have been extended to modified RNA, experimentally or bioinformatically. Here, LC-MS technologies that take RNA modifications into account when characterizing RNA-protein interactions will be developed. In addition to extensive experimental Analyses of RNA and RNA-protein complexes, this also includes the screening of datasets from public repositories to systematically include RNA modifications.

• DFG Programme: CRC/Transregios
• Subproject of: TRR 319: RMaP: RNA Modification and Processing
• Applicant Institution: Johannes Gutenberg-Universität Mainz
• Project Heads: Dr. Falk Butter; Professor Dr. Mark Helm; Professor Dr. Andres Jäschke