Molecular insights into the role of MTREC in ncRNA processing and degradation (B03)

• Subject Area: Structural Biology
• Term: since 2021
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 439669440

In eukaryotic cells, RNAs arising from pervasive transcription are rapidly degraded in the nucleus by the RNA exosome, aided by conserved adaptor complexes, such as the MTREC (Mtl1-Red1 core) complex. Here, the structure and function of MTREC will be studied by crystallography and cryo-EM. Structure and interplay with RNA substrates will be determined at the 5’-cap and the poly(A) tail, with a focus on effects of poly(A) polymerase Pla1 on poly(A) tail length. Furthermore, whether/which specific post-transcriptional RNA modifications lead to recruitment of MTREC will be investigated.

• DFG Programme: CRC/Transregios
• Subproject of: TRR 319: RMaP: RNA Modification and Processing
• Applicant Institution: Johannes Gutenberg-Universität Mainz
• Project Head: Professorin Dr. Irmgard Sinning