Dynamics of modifications and processing of RNAs in the flavivirus replication cycle (A05)

• Subject Area: Virology
• Term: since 2021
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 439669440

RNA modifications in RNA viruses are linked to RNA processing, degradation, structure formation, and in particular, immune evasion. Despite their obvious importance, results on detection and mapping of viral RNA modifications are sketchy and in part controversial. Here, flaviviral genome RNA (gRNA) modification patterns upon infection of various host cells will be analysed in detail. Modification patterns will then be analyzed in a time-resolved manner, and their impact on gRNA processing, as well as on innate immune response will be assessed. Finally, comparisons will be drawn to other RNA viruses, including Sars-CoV-2.

• DFG Programme: CRC/Transregios
• Subproject of: TRR 319: RMaP: RNA Modification and Processing
• Applicant Institution: Johannes Gutenberg-Universität Mainz
• Project Heads: Professor Dr. Mark Helm; Alessia Ruggieri, Ph.D.