Impact of RNA modifications on RNA processing and stimulation of innate immune Toll-like receptors 7/8 (A03)

• Subject Area: General Genetics and Functional Genome Biology
• Term: since 2021
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 439669440

Innate immune responses via endosomal Toll-like receptors (TLR) 7 and 8 are stimulated by exogenous microbial RNA. RNA-TLR interaction relies on RNA processing and is impeded by certain RNA modifications. Here, omics techniques will be used to study changes in subcellular proteomes, secretomes, and protein binding to immune-stimulatory RNA. The role of RNase 6 in microbial RNA processing, its cleavage mechanisms, and the role of inhibitory RNA 2’-O-ribose methylation will be analysed and further endosomal RNA interactors involved in RNA processing and TLR stimulation identified.

• DFG Programme: CRC/Transregios
• Subproject of: TRR 319: RMaP: RNA Modification and Processing
• Applicant Institution: Johannes Gutenberg-Universität Mainz
• Project Heads: Dr. Falk Butter; Professor Dr. Alexander Dalpke