Development, characterization and application of nanoprobes for advanced FLIM studies of synthetic and cellular hydrogels (A04)

• Subject Area: Biophysics
• Term: since 2021
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 431232613

In muco-obstructive diseases, the mucus layer on airway epithelial cells has an abnormally high viscosity. Using fluorescent molecular rotor (FMR) probes on samples from human donors, we observed a remarkable spatial heterogeneity of viscosity. Now, we are developing FMRs based on multi-boronated peptides that exhibit high affinity for glycans of the mucus layer and the glycocalyx. Investigations using synthetic hydrogels will dissect the impact of hydrogel rheology, mesh size, and the presence of biomacromolecules on FMR photodynamics. FLIM and ratiometric FMR probes will visualize changes in viscosity during disease and therapeutic intervention.

• DFG Programme: Collaborative Research Centres
• Subproject of: SFB 1449: Dynamic Hydrogels at Biointerfaces
• Applicant Institution: Freie Universität Berlin
• Project Heads: Dr. Stephan Block, since 1/2024; Professor Dr. Oliver Seitz