Structure, dynamics and interactions of proteins during membrane association and dissocia-tion studied by hp-FRET (A08)

• Subject Area: Biophysics
• Term: since 2016
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 267205415

In this project we want to decipher how structural identity and molecular interactions between membrane proteins and lipids give rise to the functionality of the system in and across membranes in space and time. We will combine Förster Resonance Energy Transfer measurements with super-resolved multi-parameter fluorescence image spectroscopy in vitro and in cells to study the dynamics of exemplary biomolecular assemblies with distinct project partners in this CRC: (1) maturation of the lipase LipA during translocation, (2) membrane shaping by the protein GABARAP and (3) oligomerization of the membrane protein receptors CD95.

• DFG Programme: Collaborative Research Centres
• Subproject of: SFB 1208: Identity and Dynamics of Membrane Systems - from Molecules to Cellular Functions
• Applicant Institution: Heinrich-Heine-Universität Düsseldorf
• Project Head: Professor Dr. Claus Seidel