Rolle reaktiver Sauerstoffspezies bei der Regulierung des MAPK-gesteuerten chemotrophen Wachstums und der Infektion durch das Pflanzenpathogen Fusarium oxysporum

• Antragstellerin: Dr. Daniela Elisabeth Nordzieke
• Fachliche Zuordnung: Stoffwechselphysiologie, Biochemie und Genetik der Mikroorganismen ; Allgemeine Genetik und funktionelle Genomforschung
• Förderung: Förderung von 2015 bis 2017
• Projektkennung: Deutsche Forschungsgemeinschaft (DFG) - Projektnummer 280327700

• Erstellungsjahr: 2017

Zusammenfassung der Projektergebnisse

Soil-inhabiting plant-interacting fungi such as the tomato pathogen Fusarium oxysporum are able to locate the roots of the host plant in the soil. Recently, it was shown that class III peroxidases secreted by roots function as stimuli to direct hyphal growth. Since peroxidases catalyze the reductive cleavage of hydrogen peroxide, the awardee examined in this project the role of fungal-derived hydrogen peroxide in peroxidase-triggered directed growth. She applied a combination of phenotypic assays, Western Blot analyses and microscopy approaches to provide new evidence that cooperative enzymatic activity of the fungal NADPH oxidase B complex and superoxide dismutases (SOD) provide hydrogen peroxide for the enzymatic activity of plant-derived peroxidases. This interplay results in activation of two fungal MAPK pathways. Further, she proposes that the tetraspanin Pls1 functions as a downstream target of peroxidase-mediated chemotropic signaling. Taken together, the results of this project provide new insights into the adaptation processes of plant-associated fungi allowing chemotropic detection of plant hosts.

Projektbezogene Publikationen (Auswahl)

• (2015) Interplay of MAPK and ROS signaling in chemotrophic growth of Fusarium oxysporum; 28th Fungal Genetics Conference and Fusarium Workshop, Asilomar, USA
  Dirschnabel D, Turrà D, Di Pietro A
  
• (2016) Hyphal chemotropism in fungal pathogenicity; Seminars in Cell & Developmental Biology 57:69-75
  Turrà D, Nordzieke D, Vitale S, El Ghalid M, Di Pietro A
  (Siehe online unter https://doi.org/10.1016/j.semcdb.2016.04.020)
• (2016) Link between ROS and MAPK signaling in peroxidase induced chemotropism of Fusarium oxysporum; Fusarium Workshop 2016, satellite meeting of the 13th European Conference on Fungal Genetics, Paris, France
  Nordzieke D
  
• (2016) The fungal NOX complex is required for chemotropic sensing of plant peroxidases; Interdisciplinary Forum on Virulence Mechanisms of Phyto- and Human-Pathogenic Fungi, Erlangen, Germany
  Nordzieke D