RNA Pol II CTD interactions with chromatin - crosstalk between the codes (A08)

• Subject Area: General Genetics and Functional Genome Biology
• Term: from 2013 to 2021
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 213249687

We have developed a combined genetic-mass spectrometric method to study the 240 potential phosphorylation sites in mammalian Pol II CTD. The approach provides access to the type and position of modification in each individual heptad-repeat of CTD. We combine this approach with engineered analog-sensitve kinases (asCdk7, asCdk8, asCdk9, asCdk12) to study heptad-specific phosphorylation of CTD in vivo. Further we study the heptad-specific binding of Mediator, Integrator and other factors to CTD. The project aims at better understanding of the complex relationship between dynamic changes in chromatin modifications and the activity of the transcription machinery.

• DFG Programme: Collaborative Research Centres
• Subproject of: SFB 1064: Chromatin Dynamics
• Applicant Institution: Ludwig-Maximilians-Universität München
• Project Head: Professor Dr. Dirk Eick