Mechanistic insights into the function of the ancient light-harvesting protein LHCSR3 in light energy dissipation in Chlamydomonas reinhardtii

• Applicant: Professor Dr. Michael Hippler
• Subject Area: Plant Biochemistry and Biophysics
• Term: from 2011 to 2019
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 196619610

• Final Report Year: 2020

Final Report Abstract

In the framework of the granted work, we revealed mechanistic insights into the role of N-terminal phosphorylation of LHCSR3. We conclude, that there is a dynamic interplay between LHCSR3 and LHCB4 phosphorylation that is aimed at controlling stability of PSII- supercomplexes and PSII repair. Our work also indicated that a decline in LHCSR3 phosphorylation and turn-over of the PSBS protein coincide with changes the partitioning of LEF and CEF. Here more work is required to understand these interconnections. Moreover, our data revealed the composition of the PSI-LHCI complex. It further identified that LHCSR3 binds to LHCA5 in the PSI-LHCI supercomplex forming a complex for efficient energy quenching under light stress conditions.

Publications

• (2018) Configuration of Ten Light-Harvesting Chlorophyll a/b Complex I Subunits in Chlamydomonas reinhardtii Photosystem I. Plant Physiol 178: 583-595
  Ozawa SI, Bald T, Onishi T, Xue H, Matsumura T, Kubo R, Takahashi H, Hippler M, Takahashi Y
  (See online at https://doi.org/10.1104/pp.18.00749)
• (2019) Light-dependent N-terminal phosphorylation of LHCSR3 and LHCB4 are interlinked in Chlamydomonas reinhardtii. Plant J 99: 877-894
  Scholz M, Gabelein P, Xue H, Mosebach L, Bergner SV, Hippler M
  (See online at https://doi.org/10.1111/tpj.14368)
• (2020) PsbS contributes to photoprotection in Chlamydomonas reinhardtii independently of energy dissipation. Biochimica et biophysica acta Bioenergetics 1861: 148183
  Redekop P, Rothhausen N, Rothhausen N, Melzer M, Mosebach L, Dulger E, Bovdilova A, Caffarri S, Hippler M, Jahns P
  (See online at https://doi.org/10.1016/j.bbabio.2020.148183)