In vivo application of polySia-based materials in a rat model of peripheral nerve regeneration - Impact on morphological and functional regeneration
Final Report Abstract
After soluble polySia demonstrated no negative but rather regeneration promoting effects in the context of peripheral nerve repair, more solid polySia based materials were proposed to be delivered to the Institut für Neuroanatomie. The materials delivered were then analyzed for their applicability as biomaterials for peripheral nerve repair. Till the end of the funding period no promising candidate material could be identified. However, recently nanosilica particles coated with polySia have been integrated into collagen matrices which were then rolled to achieve nerve conduits (Institut für Anorganische Chemie). Further experiments will be performed in cooperation of the Institut für Anorganische Chemie and the Institut für Neuroanatomie. The study design includes short and long term studies as follows: 1) over a four week period the nerve regeneration distance across a 10mm nerve defect will be evaluated with histological methods in 3 groups A) pure collagen tubes, B) collagen tubes infiltrated with plain nanosilica particles and C) collagen tubes infiltrated with polySia coated nanosilica particles. In parallel functional recovery will be analysed in addition to nerve histomorphometry over a period of 12 weeks in the same groups plus a D) control group transplanted with an autologous nerve graft. In case group B) and or C) demonstrate good regenerative potential the study will be advanced by 13mm nerve gap repair and monitoring of functional and axonal regeneration over a maximum of 17 weeks. The study will elucidate if incorporation of nanosilica particles coated with polySia represent a nerve regeneration promoting building block of artifical nerve grafts. To our knowledge this will be the first study on the effects of polySia immobilized within nerve conduits.
Publications
- (2008) In vivo evaluation of polysialic acid (polySia) as part of biohybrid nerve transplants. 59. Jahrestagung der Deutschen Gesellschaft für Neurochirurgie (DGNC) & 3rd Joint Meeting with the Italian Neurosurgical Society (SINch), 1.-4. Juni 2008, Würzburg, Deutschland
Haastert K, Haile Y, Rode B, Stummeyer K, Gerardy-Schahn R, Scheper T, Grothe C
- In vivo evaluation of polysialic acid (polySia) as part of bio-engineered nerve transplants. Kolloquium des Zentrums für Systemische Neurowissenschaften (ZSN) Hannover 2008, Braunlage, Germany
Schaper-Rinkel J, Grothe C and Haastert K
- In vivo evaluation of polysialic acid (polySia) as part of bioengineered peripheral nerve transplants. 38th Annual meeting of the Society for Neuroscience (SfN), Washington/DC, USA, 15.-19.11.2008
Haastert K, Haile Y, Rode B, Stummeyer K, Gerardy-Schahn R, Scheper T, Grothe C
- In vivo evaluation of polysialic acid (polySia) effects on peripheral nerve regeneration. Brainstorming IV-Kongress des Zentrums für Systemische Neurowissenschaften (ZSN) Hannover 2008, Hannover, Germany
Schaper-Rinkel J, Haile Y, Rode B, Gerardy-Schahn R, Scheper T, Grothe C and Haastert K
- In vitro- und In vivo-Untersuchungen therapeutischer Konzepte zur Förderung der anatomischen und funktionellen Regeneration über grpße Defektstrecken im nervus ischiadicus der adulten Ratte. Tierärztliche Hochschule Hannover, aus dem Institut für Neuroanatomie, Medizinische Hochschule Hannover
Ruth Schmitte
- In vivo effects of exogenous polysialic acid on peripheral nerve regeneration across nerve gaps. 26. Arbeitagung der Anatomischen Gesellschaft, Würzburg, Deutschland, 23.-25.09.2009
Schaper-Rinkel J, Schmitte R, Rode B, Gerardy-Schahn R, Scheper T, Grothe C, Haastert K
- In vivo effects of exogenous polysialic acid on peripheral nerve regeneration across nerve gaps. Kolloquium des Zentrums für Systemische Neurowissenschaften (ZSN) Hannover 2009, Braunlage, Germany
Schaper-Rinkel J, Schmitte R, Rode B, Gerardy-Schahn R, Scheper T, Grothe C and Haastert K
- In vivo evaluation of polysialic acid as candidate substance for the development of new nerve graft materials. International Symposium on Peripheral Nerve Repair and Regeneration, Turin, Italy, 04.-05.12.2009
Grothe C, Schaper-Rinkel J, Rode B, Gerardy-Schahn R, Scheper T, Haastert K
- „Intraoperative Elektrostimulation und Einsatz von PolySialin-Säure - experimentelle Ansätze für die Rekonstruktion großer Nervenlücken“. Gemeinsame Jahrestagung Sektion Schmerz und Sektion periphere Nerven der Deutschen Gesellschaft für Neurochirurgie (DGNC), Seesen (Harz), Germany, 20.02.2009
Haastert K
- (2010) In vivo evaluation of polysialic acid as part of tissue engineered nerve transplants. Tissue Eng Part A 16:3085-3098
Haastert-Talini, K., Schaper-Rinkel, J., Schmitte, R., Rode, B., Mühlenhoff, M., Schwarzer, D., Dräger, G., Su, Y., Scheper, T., Gerardy-Schahn, R.,and Grothe, C.
(See online at https://doi.org/10.1089/ten.tea.2010.0180) - Cell-based strategies to increase peripheral nerve regeneration across long gaps. Workshop W02: Strategies for promoting peripheral nerve regeneration, 7th FENS Forum of European Neuroscience, Amsterdam, The Netherlands, 03.- 07.07.2010
Haastert-Talini K, Schmitte R, Korte N, Schaper-Rinkel J, Gerardy-Schahn R., Just I, Huelsenbeck SC, Grothe C
- Förderung der peripheren Nervenregeneration durch "Tissue engineering" - In vivo-Applikation von PolySia-basierten Biomaterialien. Tierärztliche Hochschule Hannover, aus dem Institut für Neuroanatomie, Medizinische Hochschule Hannover und dem Zentrum für systemische Neurowissenschaften Hannover
Janett Kreth, geb. Schaper-Rinkel
- Incorporation of polysialic acid into tissue engineering nerve grafts: effects on peripheral nerve regeneration. SialoGlyco 2010, Postdam, Germany, 21.-26.08.2010
Haastert-Talini K, Schaper-Rinkel J, Gerardy-Schahn R, Grothe C
- Einsatz der Schwann-Zell-basierten ex vivo-Gentherapie zur Verbesserung der Regeneration peripherer Nerven nach Substanzverlust – In vitro- und in vivo-Studien am Rattenmodell. Tierärztliche Hochschule Hannover, aus dem Institut für Neuroanatomie, Medizinische Hochschule Hannover und dem Zentrum für systemische Neurowissenschaften Hannover
Kirsten Haastert
