Supplying plant peroxisomes with energy
Final Report Abstract
In this project we demonstrated that plant peroxisomes depend on the external ATP supply for the synthesis of IPP via the Mev pathway. Using our Arabidopsis mutant defective in the peroxisomal ATP import we are able to assess the contribution of plant peroxisomes for the synthesis of sterols, which will be published soon in a peer-reviewed plant science journal. During our study of the transport mechanisms of the peroxisomal ATP carrier in Arabidopsis we could not confirm a coupled co-transport with protons or pyrophosphate. Nevertheless, this unexpected outcome is leading us now to screen for a peroxisomal phosphate transporter in the future. One task of this proposal was the in vivo study of the peroxisomal ATP pool. Due to the difficulties for a functional expression of the peroxisomal ATP sensor in Arabidopsis, we currently establish a split variant of this sensor for plant expression. Such a system will facilitate a correct assembly of the fluorescent sensor inside peroxisomes and thus allow us to measure the peroxisomal ATP level in a future study. At present we analyze peroxisomal carrier proteins that might be involved in the transport of ATP or ATP-related molecules, such as CoA. Together with our cooperation partner we identified these candidates. We currently perform in vitro uptake experiments to determine their transport substrate spectrum. These preliminary results will be used to submit a new DFG project.
Publications
- (2017) Analysis of peroxisomal βoxidation during storage oil mobilization in Arabidopsis thaliana seedlings. Methods Mol Biol 1595: 291-304
Hielscher B, Charton L, Mettler-Altmann T, Linka N
(See online at https://doi.org/10.1007/978-1-4939-6937-1_27) - (2019) Plant peroxisomal solute transporter proteins. J Integr Plant Biol 61: 817-835
Charton L, Plett A, Linka N
(See online at https://doi.org/10.1111/jipb.12790) - (2020) Peroxisomal cofactor transport. Biomolecules 10: 1174
Plett A, Charton L, Linka N
(See online at https://doi.org/10.3390/biom10081174) - (2020) Slc25a17 acts as a peroxisomal coenzyme A transporter and regulates multiorgan development in zebrafish. J Cell Physiol 235: 151-165
Kim YI, Nam IK, Lee DK, Bhandari S, Charton L, Kwak S, Lim JY, Hong K, Kim SJ, Lee JN, Kwon SW, So HS, Linka N, Park R, Choe SK
(See online at https://doi.org/10.1002/jcp.28954)
