Entwicklungspotenzial, epigenetischer Status, Proteomprofil und Gesundheit von Mammalieroozyten und Embryonen aus Cryopreservierung und verbesserter und mehrstufiger Follikelkultur

• Applicants: Dr. Georg Josef Arnold; Professorin Dr. Ursula Eichenlaub-Ritter; Professor Dr. Georg Griesinger; Professor Dr. Thomas Haaf
• Subject Area: Reproductive Medicine, Urology
• Term: from 2008 to 2016
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 58733678

• Final Report Year: 2017

Final Report Abstract

Our studies provided major insight into safety and improvements in oocyte in vitro maturation, hormonal stimulation and follicle culture and cryopreservation and risk factors exposing oocytes to epimutation and changes in gene expression. Identification of markers of oocyte quality and supplementary factors that may improve cryotolerance and preimplantation development should aid in further improvement in oocyte quality and developmental capacity in oocyte IVM in animal models and in human ART.

Publications

• (2011) Evidence that carbonyl stress by methylglyoxal exposure induces DNA damage and spindle aberrations, affects mitochondrial integrity in mammalian oocytes. Hum Reprod 26:1843-59
  Tatone C, Heizenrieder T, Di Emidio G, Treffon P, Amicarelli F, Seidel T, Eichenlaub-Ritter U
  (See online at https://doi.org/10.1093/humrep/der140)
• Limiting dilution bisulfite (pyro)sequencing reveals parent-specific methylation patterns in single early mouse embryos and bovine oocytes.
  El Hajj N, Trapphoff T, Linke M, May A, Hansmann T, Kuhtz J, Reifenberg K, Heinzmann J, Niemann H, Daser A, Eichenlaub-Ritter U, Zechner U, Haaf T
  
• (2012) Oocyte ageing and its cellular basis. Int J Dev Biol 56:841-52
  Eichenlaub-Ritter U
  (See online at https://doi.org/10.1387/ijdb.120141ue)
• (2012) Vitrification at the preantral stage transiently alters inner mitochondrial membrane potential but proteome of in vitro grown and matured mouse oocytes appears unaffected. Hum Reprod 27:1096-111
  Demant M, Trapphoff T, Fröhlich T, Arnold GJ, Eichenlaub-Ritter U
  (See online at https://doi.org/10.1093/humrep/der453)
• (2013) Chronic exposure to a low concentration of bisphenol A during follicle culture affects the epigenetic status of germinal vesicles and metaphase II oocytes. Fertil Steril 100:1758-67
  Trapphoff T, Heiligentag M, El Hajj N, Haaf T, Eichenlaub-Ritter U
  (See online at https://doi.org/10.1016/j.fertnstert.2013.08.021)
• (2013) Epigenetic disturbances in in vitro cultured gametes and embryos: implications for human assisted reproduction. Fertil Steril 99:632-641
  El Hajj N, Haaf T
  (See online at https://doi.org/10.1016/j.fertnstert.2012.12.044)
• (2013) The impact of ovarian stimulation on the expression of candidate reprogramming genes in mouse preimplantation embryos. Cytogenet Genome Res 139:71-79
  Linke M, May A, Reifenberg K, Haaf T, Zechner U
  (See online at https://doi.org/10.1159/000343755)
• (2014) Dicarbonyl stress and glyoxalases in ovarian function. Biochem Soc Trans 42:433-8
  Tatone C, Eichenlaub-Ritter U, Amicarelli F
  (See online at https://doi.org/10.1042/BST20140023)
• (2014) Effects of supra-zero storage on human ovarian cortex prior to vitrification-warming. Reprod Biomed Online 29:251-8
  Klocke S, Tappehorn C, Griesinger G
  (See online at https://doi.org/10.1016/j.rbmo.2014.03.025)
• (2014) Human in vitro oocyte maturation is not associated with increased imprinting error rates at LIT1, SNRPN, PEG3 and GTL2. Hum Reprod 29:1995-2005
  Kuhtz J, Romero S, De Vos M, Smitz J, Haaf T, Anckaert E
  (See online at https://doi.org/10.1093/humrep/deu155)
• (2014) Pre- and postovulatory aging of murine oocytes affect the transcript level and poly(A) tail length of maternal effect genes. PLoS One 9:e108907
  Dankert D, Demond H, Trapphoff T, Heiligentag M, Rademacher K, Eichenlaub-Ritter U, Horsthemke B, Grümmer R
  (See online at https://doi.org/10.1371/journal.pone.0108907)
• (2015) Bisphenol A effects on mammalian oogenesis and epigenetic integrity of oocytes: a case-study exploring risks of endocrine disrupting chemicals. BioMed Research International
  Eichenlaub-Ritter U., Pacchierotti F
  (See online at https://doi.org/10.1155/2015/698795)
• (2015) Slow-freezing versus vitrification for human ovarian tissue cryopreservation. Arch Gynecol Obstet 291:419-26
  Klocke S, Bündgen N, Köster F, Eichenlaub-Ritter U, Griesinger G
  (See online at https://doi.org/10.1007/s00404-014-3390-6)
• (2016) Improved cryotolerance and developmental potential of in vitro and in vivo matured mouse oocytes by supplementing with a glutathione donor prior to vitrification. Mol Hum Reprod 22:867-881
  Trapphoff, T., Heiligentag M., Simon J., Staubach N., Seidel, T., Otte, K., Fröhlich T., Arnold G.J., Eichenlaub-Ritter, U
  (See online at https://doi.org/10.1093/molehr/gaw059)
• (2016) Postovulatory aging affects dynamics of mRNA, expression and localization of maternal effect proteins, spindle integrity and pericentromeric proteins in mouse oocytes. Hum Reprod 31:133-149
  Trapphoff T, Heiligentag M, Dankert D, Demond H, Deutsch D, Fröhlich T, Arnold GJ, Grümmer R, Horsthemke B, Eichenlaub-Ritter U
  (See online at https://doi.org/10.1093/humrep/dev279)
• (2016) Preovulatory aging in vivo and in vitro affects maturation rates, abundance of selected proteins, histone methylation pattern and spindle integrity in murine oocytes. PLoS One 11:e0162722
  Demond H, Trapphoff T, Dankert D, Heiligentag M, Grümmer R, Horsthemke B, Eichenlaub-Ritter U
  (See online at https://doi.org/10.1371/journal.pone.0162722)