Reprogramming and somatic memory in ES cell/hematopoietic stem cell hybrids

Antragsteller Professor Dr. Martin Zenke
Fachliche Zuordnung Zellbiologie
Förderung Förderung von 2008 bis 2016
Projektkennung Deutsche Forschungsgemeinschaft (DFG) - Projektnummer 66066327
 

Projektergebnisse

Erstellungsjahr 2014

Zusammenfassung der Projektergebnisse

In this project we studied two reprogramming strategies for generating pluripotent cells: (i) cell fusion with ES cells (or iPS cells) and (ii) transduction with reprogramming transcription factors to generate iPS cells. First, ES cells or iPS cells were fused with hematopoietic stem cells (HSC) to generate ES cell hybrids or iPS cell hybrids, respectively. We fund that HSC impact on differentiation kinetics and propensity of ES cell hybrids and iPS cell hybrids, which we attribute to somatic memory. Mechanistically, enhanced NODAL signaling in iPS cell hybrids caused differentiation efficiently towards mesendodermal lineages. Second, we generated iPS cells from immune-privileged Sertoli cells of testis (Ser-iPS cells). Ser-iPS cells showed reduced immunogenicity in vitro and in vivo, which we attribute to somatic memory. We suggest that taking advantage of somatic memory in reprogrammed pluripotent cells is an effective strategy for influencing their differentiation potential and biological activity.

Projektbezogene Publikationen (Auswahl)

DFG-Verfahren Schwerpunktprogramme
Teilprojekt zu SPP 1356:  Pluripotency and Cellular Reprogramming