Brain iron deposition in neurodegenerative disorders examined by MRI T2 relaxometry, pathology and biochemistry: comparative study
Final Report Abstract
21.1 Tesla Magnet Resonance Microscopy (MRM) in Neurodegeneration: OBJECTIVE: To determine if Magnetic Resonance Microscopy (MRM), which takes advantage of high resolution MRI performed at high magnetic fields, can provide unique anatomical and pathological information about neurodegenerative disease. BACKGROUND: MRM can provide high resolution 3D digital datasets based solely on endogenous contrast mechanisms that are sensitive to factors such as iron content and white fiber density. DESIGN/METHODS: Using a 33-mm birdcage coil, T2- and T2*-weighted proton MRM images at a isotropic resolution of at least 100x100x500 micron were acquired at 21.1 T. In comparison to healthy controls, excised substantia nigra (SN), globus pallidus (GP) and hippocampal (HIPP) sections taken from 21 human autopsies expressing different degrees of Alzheimer's or Parkinsonian pathology were scanned. T2 and T2* relaxation curves were fitted by exponential regression using both manually drawn ROIs placed on specific structures and a pixel-by-pixel analysis for parametric mapping. RESULTS: Compared to controls, 3D micrographs of neurodegeneration display heterogeneity in MRM contrast that appears related to either iron distribution or alterations in tissue microstructure. Pyramidal tracts of the brainstem displayed T2/T2* increases for all pathologies compared to controls. The basal ganglia's putamen showed decreased T2 while the external GP showed decreased T2* for all pathologies. Parametric maps displayed differences between pathologies. CONCLUSIONS/RELEVANCE: We present the first MRM evaluations of human pathological tissue at 21.1 T, the highest magnetic field available for MRI. Because of its specificity and spatial resolution, histological and immunological staining continues to be the standard for pathological evaluation. However, MRM offers additional complementary information that is disease specific and possibly elucidates severity. Histological evaluations are underway to verify the origins of MRM contrast. This ultra-high field strength provides sensitivity and contrast based on differences in magnetic susceptibility between tissue types and pathologies.
Publications
- Mössbauer Studies of Pathological Brain Tissues Affected by PSP Disease. Acta physica polonica A No.2, Proceedings of the XLII Zakopane School of Physics, Zakopane (2008)
J. Galazka-Friedman, E.R. Bauminger, K. Szlachta, K. Schweitzer, Z. Wszolek, D. Dickson and A. Friedman
- Provisional Patent: MMV-2008-067: System and Method for identifying the potential for and the presence of Neurodegenerative Diseases
D.F. Broderick, D.W. Dickson, Z.K. Wszolek, R. Uitti, K. Schweitzer, Uwe Klose, Daniela Berg, T. Gasser
- 2009, 134th Annual Meeting of the American Neurological Association- Movement Disorders Special Interest Group Meeting. ‘21.1 Tesla Magnet Resonance Microscopy (MRM) in Neurodegeneration’
- 21.1 Tesla Magnet Resonance Microscopy (MRM) in Neurodegeneration. Annals of Neurology (2009), Vol 66/Suppl.3, S50, T-74
Katherine J. Schweitzer, Parastou Foroutan, Dennis W. Dickson, Tä B. Ahn, Uwe Klose, Daniela Berg, Samuel C. Grant, and Zbigniew K. Wszolek
- Brainstem atrophy on routine MR study in pallidopontonigral degeneration. J Neurol. 2009 May;256(5):827-9. Epub 2009 Mar 1
Slowinski JL, Schweitzer KJ, Imamura A, Uitti RJ, Strongosky AJ, Dickson DW, Broderick DF, Wszolek ZK
- 2010, 62nd Annual Meeting of the American Academy of Neurology. ‘Future of Neuroscience Conference: Frontiers in Neuroimaging: Coming Soon to a Scanner Near You’ -’21.1 T MRM in Neurodegeneration’. Toronto, Canada
- 21.1 T MRM in Neurodegeneration. 61th Annual Meeting of the AAN, Future of Neuroscience Conference: Frontiers in Neuroimaging: Coming Soon to a Scanner Near You Neurology (2010), Vol. 74/Suppl.2, A588, SC01.003
Katherine J. Schweitzer, Parastou Foroutan, Dennis W. Dickson, Tä B. Ahn, Uwe Klose, Daniela Berg, Samuel C. Grant, and Zbigniew K. Wszolek
- A novel approach to dementia: High Resolution 1H MRI of the Human Hippocampus Performed at 21.1 T Neurology. 2010 May 18;74(20):1654
KJ Schweitzer, P Foroutan, DW Dickson, DF Broderick, U Klose, D Berg, ZK Wszolek, SC Grant
- High Resolution 1H MRI of Postmortem Human Brain Sections Performed at 21.1 T. Joint Annual Meeting ISMRM-ESMRMB; MAY 01-07, 2010; Stockholm, Sweden. Proc. Intl. Soc. Mag. Reson. Med. (2010)2010
Parastou Foroutan, Katherine J. Schweitzer, Dennis W. Dickson, Uwe Klose, Daniela Berg, Samual C. Grant, and Zbigniew K. Wszolek
- Iron and reactive oxygen species activity in parkinsonian substantia nigra. Parkinsonism Relat Disord. 2010 Jun;16(5):329-33. Epub 2010 Mar 9
Wypijewska A, Galazka-Friedman J, Bauminger ER, Wszolek ZK, Schweitzer KJ, Dickson DW, Jaklewicz A, Elbaum D, Friedman A
- An Assessment of Neurodegenerative Disorders with High-Field Magnetic Resonance Imaging (21.1T). Neurology (2011), Vol.76/Suppl 2, P04.084
Shinsuke Fujioka, Parastou Foroutan, Katherine J. Schweitzer, Melissa E. Murray, Samuel C. Grant, Dennis W. Dickson, Zbigniew K. Wszolek
- An assessment of –synucleopathy and tauopathy with high-field magnetic resonance imaging (21.1T)- new insights. Book of Abstracts, 2011 Meeting of the Genetic Epidemiology of Parkinson’s Disease Consortium 2011; page 2
Fujioka S, Murray ME, Foroutan P, Schweitzer KJ, Dickson DW, Grant SC, Wszolek ZK
- High Field MR Microscopy of Progressive Supranuclear Palsy in the Ex Vivo Human Globus Pallidus. Annual Meeting ISMRM; Montreal, Canada Proc. Intl. Soc. Mag. Reson. Med. (2011)2011;Vol. 19, p. 2387
P. Foroutan, M.E. Murray, S. Fujioka, K.J. Schweitzer, D.W. Dickson, S.C. Grant, and Zbigniew K. Wszolek
- Magnetic resonance imaging with 21.1 T and pathological correlations--diffuse Lewy body disease]. Rinsho Shinkeigaku. 2011 Aug;51(8):603-7
Fujioka S, Murray ME, Foroutan P, Schweitzer KJ, Dickson DW, Grant SC, Wszolek ZK
- T2 Signal of the hippocampus and temporal lobe white matter in the differential diagnosis of Alzheimer’s disease and lewy body disease. Neurodegenerative Diseases 2011, Volume 8, Suppl. 1, 10th AD/PD-Meeting 2011
M.E. Murray, P. Foroutan, K.J. Schweitzer, S. Fujioka, D.W. Dickson, S.C. Grant, Z.K. Wszolek
