SFB 629:  Molecular Cell Dynamics: Subcellular and Cellular Movements

• Subject Area: Biology ; Medicine
• Term: from 2003 to 2015
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 5485315

• Final Report Year: 2015

Final Report Abstract

The CRC 629 Molecular Cell Dynamics started with the aim to strengthen connections between cell biology and developmental biology to foster the understanding of how intracellular movements are coupled to cellular movements. We have discussed our progress in regular annual meetings with colleagues from all over the world and over the years significantly strengthened the scientific environment in Münster fostering the development of focussed research structures. We are confident that the CRC 629 has accomplished his mission. We could publish a series of distinguished scientific results. These included the development of new imaging technologies and important studies on membrane and cytoskeleton dynamics in the transition phase from polar to migrating cells. Moreover, the comparison of the migratory behavior of different cell types in several animal species provided insights on evolutionary conserved mechanisms. The international scientific visibility and the attractiveness of the University of Münster greatly improved through the activities of the CRC 629. Several members of the CRC 629 were recruited to other universities and new colleagues could be integrated into the CRC 629. The manifold interactions with the CRC 629 sparked the development of additional successful initiatives such as the establishment of the excellence cluster called Cells in Motion. Within the excellence cluster, the original theme of the CRC 629 is placed into a broader context including other natural scientists and translational research of medically oriented colleagues. The CRC 629 thus acted as a structure-forming element within the University of Münster.

Publications

• (2005). Abi activates WASP to promote sensory organ development. Nat Cell Biology 7, 977-84
  Bogdan S, Stephan R, Löbke C, Mertens A, Klämbt C
  
• (2005). Annexins: linking Ca2+ signalling to membrane dynamics. Nat Rev Mol Cell Biol 6, 449–461
  Gerke, V., Creutz, C.E., and Moss, S.E.
  
• (2005). Dual Functional Activity of Semaphorin 3B Is Required for Positioning the Anterior Commissure. Neuron, 48, 63–75. 3
  Julien, F., Bechara, A., Fiore, R., Nawabi, H., Zhou, H., Hoyo-Becerra, C., Bozon, M., Rougon, G., Grumet, M., Püschel, A.W., Sanes, J.R., Castellani, V.
  
• (2006). The splicing factor crooked neck associates with the RNA-binding protein HOW to control glial cell maturation in Drosophila. Neuron 52, 969–980
  Edenfeld, G., Volohonsky, G., Krukkert, K., Naffin, E., Lammel, U., Grimm, A., Engelen, D., Reuveny, A., Volk, T., and Klämbt, C.
  
• (2007). Semaphorin-1a Controls Receptor Neuron-Specific Axonal Convergence in the Primary Olfactory Center of Drosophila. Neuron 53, 169–184
  Lattemann, M., Zierau, A., Schulte, C., Seidl, S., Kuhlmann, B., and Hummel, T.
  
• (2008). Control of Chemokine-Guided Cell Migration by Ligand Sequestration. Cell 132, 463-473
  Boldajipour, B., Mahabaleshwar, H., Kardash, E., Reichman-Fried, M., Blaser, H., Minina, S., Wilson, D., Xu, Q. and Raz, E.
  
• (2008). Dual lipid modification determines the localization and PM targeting of Ca2+-regulated CBL/CIPK complexes. Plant Cell 20, 1346-1362
  Batistič, O., Schültke, S., Sorek, N., Yalovsky, S., and Kudla, J.
  
• (2008). Temporal identity in axonal target layer recognition. Nature 456, 800–803
  Petrovic, M., and Hummel, T.
  
• (2008). VE-PTP maintains the endothelial barrier via plakoglobin and becomes dissociated from VE-cadherin by leukocytes and by VEGF. J Exp Med 205, 2929-2945
  Nottebaum, A.F., Cagna, G., Winderlich, M., Gamp, A.C., Linnepe, R., Polaschegg, C., Filippova, K., Lyck, R., Engelhardt, B., Kamenyeva, O., Bixel, M.G., Butz, S., and Vestweber, D.
  
• (2009). Drosophila Cip4/Toca-1 integrates membrane trafficking and actin dynamics through WASP and SCAR/WAVE. Curr Biol. 19, 1429-37
  Fricke R, Gohl C, Dharmalingam E, Grevelhörster A, Zahedi B, Harden N, Kessels M, Qualmann B, Bogdan S
  
• (2009). Modes and regulation of glial migration in vertebrates and invertebrates. Nat Rev Neurosci 10, 769–779
  Klämbt, C.
  
• (2009). Phospholipase D1 is specifically required for regulated secretion of von Willebrand factor from endothelial cells. Blood 113, 973–980
  Disse, J., Vitale, N., Bader, M.F., and Gerke, V.
  
• (2009). Switch in FGF signalling initiates glial differentiation in the Drosophila eye. Nature 460, 758–761
  Franzdóttir, S.R., Engelen, D., Yuva-Aydemir, Y., Schmidt, I., Aho, A., and Klämbt, C.
  
• (2009). VE-PTP controls blood vessel development by balancing Tie-2 activity. J Cell Biol 185, 657-671
  Winderlich, M., Keller, L., Cagna, G., Broermann, A., Kamenyeva, O., Kiefer, F., Deutsch, U., Nottebaum, A.F., and Vestweber, D.
  
• (2010) Multiple pilus motors cooperate for persistent bacterial movement in two dimensions. Phys Rev Lett 104, 178104
  Holz, C., Opitz, D., Greune, L., Kurre, R., Koomey, M., Schmidt, M. A., and Maier, B.
  
• (2010) The motorized RhoGAP myosin IXb (Myo9b) controls cell shape and motility. Proc. Natl. Acad. Sci. USA 107, 12145-12150
  Hanley PJ, Xu Y, Kronlage M, Grobe K, Schön P, Song J, Sorokin L, Schwab A, Bähler, M
  
• (2010). A role for Rho GTPases and cell–cell adhesion in single-cell motility in vivo. Nature Cell Biology 12, 47-53
  Kardash, E., Reichman-Fried, M., Maître, JL., Boldajipour, B., Papusheva, E., Messerschmidt, EM., Heisenberg, CP., Raz, E.
  
• (2010). APC/C(Fzr/Cdh1)-dependent regulation of cell adhesion controls glial migration in the Drosophila PNS. Nat Neurosci 13, 1357–1364
  Silies, M., and Klämbt, C.
  
• (2010). Ephrin-B2 controls VEGF-induced angiogenesis and lymphangiogenesis. Nature 465, 483-486
  Wang, Y., Nakayama, M., Pitulescu, M.E., Schmidt, T.S., Bochenek, M.L., Sakakibara, A., Adams, S., Davy, A., Deutsch, U., Lüthi, U., Barberis, A., Benjamin, L.E., Mäkinen, T., Nobes, C.D., and Adams R.H.
  
• (2010). Regulation of Developmental Lymphangiogenesis by Syk+ Leukocytes. Developmental Cell 18, 437–449
  Böhmer, R., Neuhaus, B., Bühren, S., Zhang, D., Stehling, M., Böck, B., and Kiefer, F
  
• (2011) Stabilizing the VE- cadherin-catenin complex blocks leukocyte extravasation and vascular permeability. Embo J 30, 4157-7410
  Schulte, D., Küppers, V., Dartsch, N., Broermann, A., Li, H., Zarbock, A., Kamenyeva, O., Kiefer, F., Khandoga, A., Massberg, S., and Vestweber, D.
  
• (2011). Activation of a RhoA/Myosin II-Dependent but Arp2/3 Complex-Independent Pathway Facilitates Salmonella Invasion. Cell Host & Microbe 9, 273–285
  Hänisch, J., Kölm, R., Wozniczka, M., Bumann, D., Rottner, K., and Stradal, T.E.B.
  
• (2011). Dendritic spine formation and synaptic function require neurobeachin. Nature Commun 2, 557
  Niesmann, K., Breuer, D., Brockhaus, J., Born, G., Wolff, I., Reissner, C., Kilimann, M.W., Rohlmann, A., and Missler, M.
  
• (2012). Altered BCR signalling quality predisposes to autoimmune disease and a pre-diabetic state. Embo J 31, 3363–3374
  Königsberger, S., Prodöhl, J., Stegner, D., Weis, V., Andreas, M., Stehling, M., Schumacher, T., Böhmer, R., Thielmann, I., van Eeuwijk, J.M.M., Nieswandt, B., Kiefer, F.
  (See online at https://doi.org/10.1038/emboj.2012.169)
• (2012). Entry of Human Papillomavirus Type 16 by actin-dependent, clathrin-, caveolin-, and lipid raft independent endocytosis. PLoS pathog. 8, e1002657
  Schelhaas, M., Shah, B., Holzer, M., Blattmann, P., Kühling, L., Day, P.M., Schiller, J.T., and Helenius, A.
  
• (2012). Identification and regulation of a molecular module for bleb-based cell motility. Developmental Cell 23, 210-218
  Goudarzi, M., Banisch, T.U., Mobin, M.B., Maghelli, N., Tarbashevich, K., Strate, I., van den Berg, J., Blaser, H., Bandemer, S., Paluch, E., Bakkers, J., Tolić-Nørrelykke, I.M., Raz, E.
  (See online at https://doi.org/10.1016/j.devcel.2012.05.007)
• (2013). A novel multistep mechanism for initial lymphangiogenesis in mouse embryos based on ultramicroscopy. Embo J 32, 629–644
  Hägerling, R., Pollmann, C., Andreas, M., Schmidt, C., Nurmi, H., Adams, R.H., Alitalo, K., Andresen, V., Schulte-Merker, S., and Kiefer, F.
  (See online at https://doi.org/10.1038/emboj.2012.340)
• (2013). Ephrin-B2 controls PDGFRß internalization and signalling. Genes Dev 27, 2576-2589
  Nakayama, A., Nakayama, M., Turner, C.J., Höing, S., Lepore, J.J., and Adams, R.H.
  
• (2013). SILAC Proteomics of Planarians Identifies Ncoa5 as a Conserved Component of Pluripotent Stem Cells. Cell Rep 5, 1142–1155
  Böser, A., Drexler, H.C.A., Reuter, H., Schmitz, H., Wu, G., Schöler, H.R., Gentile, L., and Bartscherer, K.
  (See online at https://doi.org/10.1016/j.celrep.2013.10.035)
• (2013). Spatial regulation of VEGF receptor endocytosis in angiogenesis. Nat Cell Biol 15, 249-260
  Nakayama, M., Nakayama, A., van Lessen, M., Yamamoto, H., Hoffmann, S., Drexler, H.C., Itoh, N., Hirose, T., Breier, G., Vestweber, D., Cooper, J.A., Ohno, S., Kaibuchi, K., and Adams R.H.
  (See online at https://doi.org/10.1038/ncb2679)
• (2014) Leukocyte extravasation and vascular permeability are each controlled in vivo by a different tyrosine residue of VE-cadherin. Nat Immunol 15, 223-230
  Wessel, F., Winderlich, M., Holm, M., Frye, M., Rivera-Galdos, R., Vockel, M., Linnepe, R., Ipe, U., Stadtmann, A., Zarbock, A., Nottebaum, A., and Vestweber, D.
  (See online at https://doi.org/10.1038/ni.2824)
• (2014). Arteries are formed by vein-derived endothelial tip cells. Nat Commun 5, 5758-5769
  Xu, C., Hasan, S.S., Schmidt, I., Rocha, S.F., Pitulescu, M.E., Bussmann, J., Meyen, D., Raz, E., Adams, R.H., and Siekmann, A.F.
  (See online at https://doi.org/10.1038/ncomms6758)
• (2014). ECM stiffness regulates glial migration in Drosophila and mammalian glioma models. Development 141, 3233–3242
  Kim, S.N., Jeibmann, A., Halama, K., Witte, H.T., Wälte, M., Matzat, T., Schillers, H., Faber, C., Senner, V., Paulus, W., and Klämbt, C.
  (See online at https://doi.org/10.1242/dev.106039)
• (2014). Modulation of synaptic function through the alpha-neurexin-specific ligand neurexophilin-1. Proc Natl Acad Sci USA 111, E1274-1283
  Born, G., Breuer, D., Wang, S., Rohlmann, A., Coulon, P., Vakili, P., Reissner, C., Kiefer, F., Heine, M., Pape, H.C., and Missler, M.
  (See online at https://doi.org/10.1073/pnas.1312112111)
• (2014). The WAVE regulatory complex links diverse receptors to the actin cytoskeleton. Cell 156, 195-207
  Chen B, Brinkmann K, Chen Z, Pak CW, Liao Y, Shi S, Henry L, Grishin NV, Bogdan S, Rosen MK
  (See online at https://doi.org/10.1016/j.cell.2013.11.048)
• (2015). Chemokine-Guided Angiogenesis Directs Coronary Vasculature Formation in Zebrafish. Developmental Cell 33, 442–454
  Harrison, M.R.M., Bussmann, J., Huang, Y., Zhao, L., Osorio, A., Burns, C.G., Burns, C.E., Sucov, H.M., Siekmann, A.F., and Lien, C.-L.
  (See online at https://doi.org/10.1016/j.devcel.2015.04.001)
• (2015). Dynamic filopodia are required for chemokine-dependent intracellular polarization during guided cell migration in vivo. eLife 2015;4:e05279
  Meyen, D., Tarbashevich, K., Banisch, T.U., Wittwer, C., Reichman-Fried, M., Maugis, B., Grimaldi, C., Messerschmidt, E., Raz, E.
  (See online at https://dx.doi.org/10.7554/eLife.05279.001)
• (2015). Impaired protein translation in Drosophila models for Charcot-Marie-Tooth neuropathy caused by mutant tRNA synthetases. Nat Commun 6, 7520
  Niehues, S., Bussmann, J., Steffes, G., Erdmann, I., Köhrer, C., Sun, L., Wagner, M., Schäfer, K., Wang, G., Koerdt, S.N., Stum, M., RajBhandary, U.L., Thomas, U., Aberle, H., Burgess, R.W., Yang, X.-L., Dieterich, D., and Storkebaum, E.
  (See online at https://doi.org/10.1038/ncomms8520)
• (2015). Vacuolar CBL-CIPK12 Ca2+ sensor-kinase complexes are required for polarized pollen tube growth. Curr. Biol. 25, 1475-1482
  Steinhorst, L., Mähs, A., Ischebeck, T., Zhang, C., Zhang, X., Arendt, S., Schültke, S., Heilmann, I., and Kudla, J.
  (See online at https://doi.org/10.1016/j.cub.2015.03.053)
• (2015). β-Catenin-Dependent Control of Positional Information along the AP Body Axis in Planarians Involves a Teashirt Family Member. Cell Rep 10, 253–265
  Reuter, H., März, M., Vogg, M.C., Eccles, D., Grífol-Boldú, L., Wehner, D., Owlarn, S., Adell, T., Weidinger, G., and Bartscherer, K.
  (See online at https://doi.org/10.1016/j.celrep.2014.12.018)