Transregio 5 is an interdisciplinary research network focused on understanding the regulatory dimension imposed on genes by their chromatin organisation. The problem is addressed at various structural levels ranging from single nucleosomes and nucleosomal fibers to chromosomal domains and entire chromosomal territories employing well-developed experimental systems and selected model organisms. This integrated approach promises novel insights into basic principles and mechanisms governing central nuclear functions in eukaryotes including gene regulation, DNA replication and the repair of damaged DNA. Major goals of the programme are
-- to determine the factors and processes involved in the positioning and posttranslational modification of nucleosomes with regulatory function;
-- to assess the functional consequences of specific chromatin structures, considering interaction of non-histone regulators, chromatin folding and nuclear compartmentalisation;
-- to explore the principles by which enzymes that modify chromatin are targeted to defined gene loci, chromosomal domains and nuclear compartments;
-- to understand the principles which govern dynamic chromatin transitions and stable epigenetic memory.
DFG Programme
CRC/Transregios
International Connection
Switzerland, United Kingdom
Completed projects
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A02 - Monitoring expression-related changes of gene topology in nuclei of living cells
(Project Heads
Brack-Werner, Ruth
;
Cremer, Thomas
)
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A4 - Role of Nuclear Matrix Attachment Regions for Gene Expression and Chromatin Accessibilty
(Project Head
Grosschedl, Rudolf
)
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A06 - Regulation of the ribosomal DNA genes in chromatin
(Project Head
Längst, Gernot M.
)
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A7 - Function of transcription coactivator complexes in chromatin
(Project Head
Meisterernst, Michael
)
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A08 - Chromatin remodelling in Xenopus development
(Project Head
Rupp, Ralph A. W.
)
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A09 - Analysis of the functional properties of chromatin domains in relation to their modifications and their compartmentalization in the cell nucleus
(Project Head
Zink, Daniele
)
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A10 - Chromatin structure and control of the rDNA locus
(Project Head
Grummt, Ingrid
)
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A11 - Identification and characterization of target genes for the Drosophila MBD2/3 protein
(Project Head
Lyko, Frank
)
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A12 - Epigenetic mechanisms maintaining transcriptional memory
(Project Head
Paro, Renato
)
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A13 - Regulation of gene expression by histone methylation and demethylation
(Project Head
Sauer, Frank
)
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A14 - Growth dependent regulation of rRNA synthesis in yeast: components of the yeast RNA polymerase I transcription machinery and their interaction with charomatin
(Project Head
Tschochner, Herbert
)
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A15 - Isolation and characterisation of mammalian MSL complex
(Project Head
Akhtar, Ph.D., Asifa
)
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A18 - Targeting chromatin-modifying protein complexes to regulatory DNA elements
(Project Head
Müller, Jürg
)
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A19 - Centromeric Chromatin
(Project Head
Schiebel, Elmar
)
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M01 - Dosage Compensation in Drosophila
(Project Head
Becker, Peter Burkhard
)
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M02 - Study of RNA polymerase II functions required for transcription of chromatin templates
(Project Head
Eick, Dirk
)
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M03 - Structure-function analysis of chromatin transcription factors
(Project Head
Cramer, Patrick
)
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M04 - Dynamic Changes of Chromatin States
(Project Head
Imhof, Axel
)
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M05 - Developmental Maturation of the Chromatin Structure
(Project Head
Rupp, Ralph A. W.
)
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M06 - Nucleosome Positioning and Remodeling in the Yeasts S. cerevisiae and S. pombe
(Project Head
Korber, Philipp
)
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M07 - Cryo-EM analysis of the ISWI chromatin remodeller ACF
(Project Head
Beckmann, Roland
)
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M08 - Functional architecture of the INO80 chromatin-remodelling complex in DNA repair
(Project Head
Hopfner, Karl-Peter
)
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M09 - Control of DNA replication by epigenetic modification and chromatin remodelling
(Project Head
Schepers, Aloys
)
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M10 - The Role of Mammalian Histone H3 Variants in Gene Regulation
(Project Head
Hake, Sandra Brigitte
)
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M11 - The biological roles of H4K20 Methylierung
(Project Head
Schotta, Gunnar
)
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M12 - Mechanism of cell cycle tegulation by histone H3 lysine 76 methylation in Trypanosoma brucei
(Project Head
Janzen, Christian
)
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M13 - Role and regulation of DNA methyltransferase interactions with chromatin
(Project Head
Leonhardt, Heinrich
)
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M15 - Kinetics of chromatin formation and CpG methylation of Epstein-Barr Virus DNA
(Project Head
Hammerschmidt, Wolfgang
)
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Z01 - Administration of the Transregio and Coordination of Activities
(Project Head
Becker, Peter Burkhard
)
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Z02 - Core Facility monoclonal antibodies fpr CRC TRR 5
(Project Head
Kremmer, Elisabeth
)
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Z03 - Protein Analysis Unit
(Project Head
Imhof, Axel
)
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Z04 - Advanced live-cell and subdiffration resolution imaging to sudy cell cycle dependent functional chromatin organization and dynamics
(Project Head
Schermelleh, Lothar
)
