Project Details
Projekt
Effects of veterinary medicines in manure on the abundance and transfer of bacterial antibiotic resistance genes in soil: importance of the rhizosphere and of prepeated manure applications
Applicant
Professorin Dr. Kornelia Smalla
Subject Area
Microbial Ecology and Applied Microbiology
Soil Sciences
Soil Sciences
Term
from 2005 to 2014
Project identifier
Deutsche Forschungsgemeinschaft (DFG) - Project number 5471428
In the first phase of this project we showed that soil fertilization with manure containing antibiotics enhanced the abundance of antibiotic resistance genes and their transferability, and identified a plasmid type that plays a major role in horizontal spread of resistance genes between manure and soil bacteria. In the second phase we aim to elucidate if the effects of soil fertilization with manure containing veterinary medicines are influenced by the rhizosphere and by repeated application of manure. This will be investigated in joint soil mesocosm and field experiments. Analysis and modeling of rhizophere effects at a low spatial and temporal resolution will be performed in microcosms (with A1, C). Accumulation of resistant populations in bulk and rhizosphere soil by repeated manure application will be investigated in samples from a central field experiment. Quantitative data on the abundance of resistance genes and plasmids in total community (TC)-DNA will be obtained by real time PCR. These data will be related to extractable and non-extractable amounts of antibiotic compound in soil to clarify their bioavailability and selective force. We aim to identify and quantify the hosts of resistance plasmids in manure and soil, in order to incorporate their habitat-specific fate and inter-species plasmid transfer into a mathematical model (C).
DFG Programme
Research Units
