Project Details
Projekt
Veterinary medicinal effects on the prokaryotic structural diversity in soil microcopartments - VMMic
Applicant
Professor Dr. Sören Thiele-Bruhn
Subject Area
Soil Sciences
Term
from 2005 to 2014
Project identifier
Deutsche Forschungsgemeinschaft (DFG) - Project number 5471428
Soil antibiotics affect adversely soil microbial biomass, activity, structural and functional diversity, and resistance level. We found in the 1st phase of FOR that antibiotic effects are strongly influenced by manure as a nutrient source and microbial inoculum. Single applications of antibiotics yield mid-term effects on the soil microbial structural diversity. However, major aspects of a field situation, relevant for the effects of antibiotics and of xenobiotics in general, have not been researched so far, i.e. influences of structured soil heterogeneity with microcompartments such as the rhizosphere, weather conditions, and repeated manuring. These aspects will be investigated in this project with special regard to a hypothesized spatial proximity and interactions of antibiotics and prokaryotes in soil microcompartments that yield an apparent concentration independency of antimicrobial effects. Structural diversity analysis of bacteria and fungi is completed by investigations on functional redundant archaea. This will be done in a threefold approach: (i) analysis of soil prokaryotic structural diversity with PLFA, GDGT, and 16s rDNA analysis; (ii) determination of spatial distribution by combined soil micromorphology, fluorescence staining and hybridisation, microscopy, and autoradiography; (iii) analysis of sulfonamide residual concentrations and dissipation kinetics.
DFG Programme
Research Units
