Functional analysis of the NF-Y transcription factor family for gene activation during adaptation to environmental stress. Exploration of properties and functions of specific members of the NF-Y family for gene activation during adaptation to environmental stress
Final Report Abstract
The project was devoted to the elucidation of the function of different subunits of the heterotrimeric transcription factor NF-Y (nuclear factor Y) during physiological stress responses. The heterotrimeric NF-Y binds to the well-known CCAAT-box motif and functions as activator in the disintegration of nucleosomal structures in yeast and mammals, so that other factors can bind in the promoter regions. While human and animals encode the three subunits of NF-Y in single copy genes, Arabidopsis thaliana possesses 10, 13 and 13 genes encoding the NF-Y subunits A, B and C, respectively. It is hypothesized that specific tasks are attributed to plant NF-Ys during development and environmental stress. These tasks are achieved by the function-dependent combinatorial assembly of individual subunits to NF-Y and subsequent binding to CCAAT-boxes of specific genes. It was the aim of the project to deliver particular experimental evidence for the role of the various subunits, such as NF- YC27, NF-YC22, NF-YA5 and NF-YB3. The T-DNA-insertion of their genes causes phenotypes of photooxidative sensitivity, in flowering time and during abiotic stress. The interactomes of all interacting NF-Y subunits were established. In further studies redoxdependent dimerisation, subcellular partitioning of NF-YB and their co-transport with NF-YC were explored.
Publications
- Studies on Differential Nuclear Translocation Mechanism and Assembly of the Three Subunits of the Arabidopsis thaliana Transcription Factor NF-Y. Mol Plant. 2011 Dec 22. [Epub ahead of print] PubMed PMID: 22199235
Hackenberg D, Wu Y, Voigt A, Adams R, Schramm P, Grimm B
- Technical note – High-throughput analysis of bilateral protein-protein interactions using the yeast two hybrid system. J of Endocytobiosis and Cell Research (2012) 6-13
Hackenberg D and Grimm B
