Gentransfer von Zytokin- und Antiapoptose-Proteinen in die Kornea: Untersuchungen zur Prävention der Transplantatabstoßung nach perforierender Keratoplastik (Hornhauttransplantation)

• Antragsteller: Professor Dr. Uwe Pleyer
• Fachliche Zuordnung: Augenheilkunde
• Förderung: Förderung von 2004 bis 2007
• Projektkennung: Deutsche Forschungsgemeinschaft (DFG) - Projektnummer 5434563

• Erstellungsjahr: 2008

Zusammenfassung der Projektergebnisse

Keine Zusammenfassung vorhanden

Projektbezogene Publikationen (Auswahl)

• Effects of adenovims-mediated gene transfer of IL-12p40 on comeal allograft survival. ARVO-meeting, Fort Lauderdale, USA, 2003. Poster
  Ritter T., Yang J., Dannowski, H., Vogt K., Hempel M., Pleyer, U.
  
• Efficiency of cytokine gene transfer in corneal endothelial cells and organ cultured corneas mediated by liposomal vehicles and recombinant adenovirus. Ophthalmic Research 2003. 35: 117-124
  Bertelmann E., Ritter, T., Vogt, K., Reszka, R., Hartmann, C , Pleyer, U.
  
• "Gene transfer of AdCTLA4-Ig prolongs experimental comeal allograft survival" EVER 24. Sept. 2004, Vilamoura, Portugal
  
  
• "Hornhauttransplantation: Anspruch und Wirklichkeit» 77. Versammlung der Vereinigung Rhein-Mainischer Augenärzte 6. November 2004, Homburg
  
  
• "Penetrating keratoplasty: current problems- current solutions and future perspectives' 7th Congress of Ophthalmologists of Serbia 28. September- 1. Oktober 2004, Belgrade, Serbia and Montenegro
  
  
• "Selective steroid receptor agonists ( SEGRA) a future therapeutic option?" EVER 26. Sept. 2004, Vilamoura, Portugal
  
  
• Blockade of co-stimulation by CTLA4Ig gene therapy improves comeal allograft survival but the effect is influenced by corneal-specific immunity. DGFI, Maastricht, 2004. Poste
  Gong, N., Yang, J., Vogt, K., Hill, M., Anegon, I., Volk, H.-D., Pleyer, U., Ritter, T.
  
• Einfluss von Cyclodextrinen auf die okulare Verfügbarkeit von Antiglaukomatosa und Immunsuppressiva in vitro/in vivo. Mathematisch- Naturwissenschaftliche Fakultät I der Humboldt-Universität Berlin, Institut fiir Pharmazie, 2004
  Sigrid Knapp
  
• Gene expression of viral Interleukin-10 (vIL-10) prolongs survival of comeal allografts depending on the way of application. ARVO-meeting, Fort Lauderdale, USA, 2004. Poster
  Gong, N., Yang, J., Dannowski, H., Ritter, T., Pleyer, U.
  
• Gene transfer of anti-apoptotic and cytoprotective molecules in human comeal endothelial cells and their influence on prevention of apoptosis. ARVO-meeting, Fort Lauderdale, USA, 2004. Poster
  Ritter, T., Ecke, I., Yang, J., Metzner, S., Schu, S., Volk, H.-D., Pleyer, U.
  
• Gentransfer in komeale Endothelzellen: nicht-virale und virale Ansätze zur Modulation der Endothelzellfunktion und allogenen Immunreaktion. Mathematisch-Naturwissenschaftliche Fakultät I der Humboldt-Universität Berlin, Institut für Biologie, 2004
  Haike Dannowski
  
• Effects of a selective steroid receptor agonist (SEGRA) on experimental keratoplasty. Graefes Arch Clin Exp Ophthalmol. 2005. 243 : 450-455
  Pleyer, U., Yang, J., Knapp, S., Schäcke H., Schmees N., Orlic, N., Otasevic, L., De Ruijter, M., Ritter, T., Keipert, S.
  
• Influence of local and systemic CTLA41g gene transfer on comeal allograft survival. J. Gene Med. 2006 Apr;8(4):459-67
  Gong, N., Pleyer, U., Yang, J., Vogt, K., Hill, M., Anegon, I., Volk, H.-D., Ritter, T.
  
• „New aspects of immune reactions after comeal grafting: The taming of the shrew" XVII. Annual Meeting of European Eye Bank Association 14.-16. Januar 2005, Halle/Saale
  
  
• „The future of comeal transplantation: Physiology and Pathophysiology and implication for comeal refractive surgery" Opening Lecture of the VII. International Congress on Refractive Surgery 2005 10. März 2005, Alicante, Spanien
  
  
• "Taming of the shrew" or: "Prevention of allograft response" B. Straatsma Award and Lecture (UCLA), 2006, Los Angeles, USA
  
  
• Influence of local and systemic CTLA4Ig gene transfer on comeal allograft survival- 7th International Conference on New Trends in Immunosuppression and Immunotherapy, Berlin, Germany, February 16 - 19, 2006. Poster
  Ritter, T. Gong, N., Yang, J., Vogt, K., Hill, M., Anegon, I., Volk, H.D., Pleyer, U.
  
• Local over-expression of nerve growth factor in comeal transplants improves allograft survival. 1st Joint Meeting of European National Societies of Immunology - 16th European Congress of Immunology, Paris 2006 Sept 6-9. Poster
  Ritter, T., Gong N., Vogt, K., Anegon, I., Flügel, A, Volk H-D, Pleyer, U.
  
• Progress in corneal concordant xenotransplantation. Prag Czec Republic, 2006
  Klara Sedlakova
  
• Risk factors for endothelial cell loss post-keratoplasty. Acta Ophthalmol Scan: 84 (6) 766-770, 2006
  Bertelmann E., Pleyer U. & Rieck P.
  
• "Gene therapy in corneal transplantation - Current status and future perspectives" Asian ARVO, 2007, Singapur
  
  
• "Novel therapeutic strategies to prevent comeal allograft rejection" lOIS 2007, Paris, Frankreich
  
  
• Current aspects ofthe immunobiology and prevention of comeal graft rejection:what have we leamed from 100 years of keratoplasty ? In: Pleyer U, Foster CS (Eds) Essentials in Ophthalmology. Uveitis and immunological disorders. Springer, Berlin, Heidelberg, pp 46-75, 2007
  X.Q. Li, S. Schlickeiser, Pleyer U
  
• Effects of Interleukin-12p40 gene transfer on rat corneal allograft survival. Transplant Immunology. 2007; 18: 101-107
  Ritter, T., Yang, J., Dannowski, H., Vogt, K., Volk, H.-D., Pleyer, U.
  
• Effects of local and systemic viral Interieukin-10 gene transfer on comeal allograft survival. Gene Ther. 2007 Mar;14(6):484-90. Epub 2006 Nov 9
  Gong, N., Pleyer, U., Volk, H.-D., Ritter, T.
  
• Effects of Spirolactone on comeal allograft survival inthe rat. Ophthalmic Res. 2007;39:325-329
  Otasevic, L., Gong, N., Ritter, T., Mergler, S., Pleyer, U.
  
• Formulation of sirolimus eye drops and corneal permeation studies. J Ocul Pharmacol Ther. 2007 Jun;23(3):292-303
  Buech G, Bertelmann E, Pleyer U, Siebenbrodt I, Borchert HH
  
• Gene transfer of cyto-protective molecules in corneal endothelial cells and cultured comeas: Analysis of protective effects in vitro and in vivo. Biochem Biophys Res Commun. 2007c May 25;357(1):302- 7. Epub 2007 Apr 2
  Gong, N., Ecke, L, Mergler, S., Yang, J., Metzner, S., Schu, S., Volk, H.-D., Pleyer, U., Ritter, T.
  
• Influence of Inducible Costimulator fusion protein (ICOSlg) gene transfer on comeal allograft survival. Graefes Arch Clin Exp Ophthalmol. 2007 Oct;245(l0):15l5-21
  Fabian, D., Gong, N., Vogt, K., Volk, H.-D., Pleyer, U., Ritter, T.
  
• Local over-expression of nerve growth factor in comeal transplants improves allograft survival. Invest. Ophthal. Vis. Sei. 2007 48: 1043-1052
  Gong, N., Pleyer, U., Vogt, K., Anegon, 1., Fluegel, A., Volk, H.-D., Ritter, T.
  
• Transient receptor potential vanilloid 1 activation induces inflammatory cytokine release in comeal epithelium through MAPK signaling. J Cell Physiol. 2007 May 16
  Zhang F, Yang H, Wang Z, Mergler S, Liu H, Kawakita T, Tachado SD, Pan Z, Capo-Aponte JE, Pleyer U, Koziel H, Kao WW, Reinach PS
  
• Western, Northern and Southern Blotting. In: Basic Science Techniques in Clinical Practice Patel, H. R. H.; Arya, M.; Shergill, I. S. (Eds.) Springer, Heidelberg, 2007
  S. Schlickeiser, Pleyer U.
  
• "Gene- and cell therapeutic applications for the prevention of corneal graft rejection" XVIII. International Congress for Eye Research (ICER 2008), Beijing, China
  
  
• "Prophylaxis of immune reactions from gene therapy" DOG Symposium, 2008, Berlin
  
  
• Corneal permeation studies of everolimus microemulsion. J Ocul Pharmacol Ther. 2008 Aug;24(4):399-402
  Baspinar Y, Bertelmann E, Pleyer U, Buech G, Siebenbrodt I, Borchert HH
  
• Ever Lecture 2008 "The taming of the shrew? 100 years of comeal transplantation" EVER, 2008, Portoroz, Slowenia
  
  
• Keratoplasty in HSV keratitis: prevention and therapy for immunological complications. Klin Monatsbl Augenheilkd. 2008 Jan;225(l):22-9
  Stanojlovic S, Schlickeiser S, Pleyer U
  
• Prolongation of Comeal Allograft Survival by Topical Application of Everolimus in Experimental Keratoplasty. Ophthalmic Res.2008 7;40(6):309-314
  Li XQ, Buch G, Otasevic L, Schlickeiser S, Bertelmann E, Pleyer U
  
• The Survival of Herpes Simplex Vims in Preserved Murine Comeas. Ophthalmic Res. 2008; 40(6):329-332
  Robert PY, Ducher V, Pleyer U, Denis F, Adenis JP, Ranger-Rogez S.