Protein-to-DNA electron transfer reactions using DNA binding enzymes

• Applicant: Dr. Alexandra Spieß
• Subject Area: Organic Molecular Chemistry - Synthesis and Characterisation
• Term: from 2004 to 2008
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 5429976

Recently electron transfer reactions can be observed between peptides or proteines and the DNA. Lys-Trp-Lys and Lys-Tyr-Lys have been as model peptides and M.Hhal was used as first example of a DNA-binding protein. The project of this research fellowship will be the investigation of DNA-mediated electron-transfer reactions modulated by the Fe-S-cluster containing enyzmes MutY, Endo III or SoxR. It is planned to observe the electron transfer process spectroscopically and analyze the resulting oxidative DNA damage by gel electrophoresis.

• DFG Programme: Research Fellowships