Functional and structural characterization of bacterial blue-light sensitive photoreceptors

• Applicant: Professor Dr. Wolfgang Gärtner
• Subject Area: Biophysics
• Term: from 2004 to 2011
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 5470629

• Final Report Year: 2012

Final Report Abstract

Im TP A1 wurden bakterielle Phototropin-ähnliche Photorezeptoren untersucht. Beginnend mit dem ersten prokaryotischen Vertreter dieser Substanzklasse, YtvA aus B. subtilis, wurden spektroskopische, kinetische und funktionelle Experimente zum Verständnis der molekularen Reaktivität und zur Erzeugung und Weiterleitung des biologischen Signals durchgeführt. Im weiteren Verlauf wurden, aufbauend auf Datenbank-Suchen, Gene für neuartige bakterielle LOV-Domänen Proteine identifiziert, kloniert und deren rekombinante Genprodukte funktionell charakterisiert. Besonderes Gewicht wurde hierbei auf die Demonstration lichtinduzierter Regulation ankondensierter Enzymaktivitäten gelegt. Abschliessend wurden die Arbeiten auf Metagenom-Analysen und funktionelle Charakterisierung möglicher LOV-Domänen aus diesen Daten ausgedehnt.

Publications

• (2004) Functional variations among LOV domains as revealed by FTIR difference spectroscopy. Photochem. Photobiol. Sci. 3 575- 579
  Bednarz, B., Losi, A., Gärtner, W., Hegemann, P. and Heberle, J.
  (See online at https://doi.org/10.1039/B400976B)
• (2004) Tryptophan fluorescence in the Bacillus subtilis phototropinrelated protein YtvA as a marker of interdomain interaction. Photochem. Photobiol. 80 150-153
  Losi, A., Ternelli, E. and Gärtner, W.
  (See online at https://doi.org/10.1111/j.1751-1097.2004.tb00063.x)
• (2005) Initial characterization of a blue-light sensing, phototropin-related protein from Pseudomonas putida: a paradigm for an extended LOV construct. PCCP 7 2804-2811
  Krauss, U., Losi, A., Gärtner, W., Jaeger, K.-E. and Eggert, T.
  (See online at https://doi.org/10.1039/B504554A)
• (2005) Mutational effects on protein structural changes and interdomain interactions in the blue-light sensing LOV protein YtvA. Photochem. Photobiol. 81 1145-1152
  Losi, A., Ghiraldelli, E., Jansen, S. and Gärtner, W.
  (See online at https://doi.org/10.1562/2005-05-25-RA-541)
• (2006) Blue news: NTP-binding properties of the blue light-sensitive YtvA protein from Bacillus subtilis. FEBS Lett. 580 3818-3822
  Buttani, V., Losi, A., Polverini, E. and Gärtner, W.
  (See online at https://doi.org/10.1016/j.febslet.2006.06.007)
• (2007) Conformational analysis of the blue-light sensing protein YtvA reveals a competitive interface for LOV-LOV dimerization and interdomain interactions. Photochem. Photobiol. Sci. 6 41-49
  Buttani, V., Losi, T., Eggert, T., Krauss, U., Jäger, K.-E., Cao, Z., and Gärtner, W.
  (See online at https://doi.org/10.1039/B610375H)
• (2007) In vivo fluorescence without oxygen: Novel fluorescence reporters for anaerobic systems. Nature Biotechnology 25 443-445
  Drepper, T., Eggert, T., Circolone, F., Heck, A., Krauß, U., Guterl, J.-K., Wendorff, M., Losi, A, Gärtner, W., Jaeger, K.-E.
  (See online at https://doi.org/10.1038/nbt1293)
• (2007) NTP-binding properties of the blue-light receptor YtvA and effects of the E105L mutation. Eur. Biophys. J. 36 831-839
  Buttani, V., Gärtner, W., Losi, A.
  (See online at https://doi.org/10.1007/s00249-007-0155-1)
• (2008) A blue light inducible two component signal transduction system in the plant pathogen Pseudomonas syringae pv. tomato. Biophys. J. 94 897-905
  Cao, Z., Buttani, V., Losi, A. and Gärtner, W.
  (See online at https://doi.org/10.1529/biophysj.107.108977)
• (2008) Bacterial bilin- and flavin-binding photoreceptors. Photochem. Photobiol. Sci. 7 1168-1178
  Losi, A. and Gärtner, W.
  (See online at https://doi.org/10.1039/B802472C)
• (2008) Shedding (blue) light on algal gene expression. Proc. Natl. Acad. Sci. USA 105 7-8
  Losi, A. and Gärtner, W.
  (See online at https://doi.org/10.1073/pnas.0710523105)
• (2009) A blue light inducible phosphodiesterase activity in the cyanobacterium Synechococcus elongatus. Photochem. Photobiol. 86 606-611
  Cao, Z., Livoti, E., Losi, A., and Gärtner, W.
  (See online at https://doi.org/10.1111/j.1751-1097.2010.00724.x)
• (2009) Distribution and Phylogeny of Light-Oxygen-Voltage-Blue-Light-Signaling Proteins in the Three Kingdoms of Life. J. Bact. 191 7234-7242
  Krauss, U., Minh, B.Q., Losi, A., Gärtner, W., Eggert, T., v. Haeseler, A., and Jäger, K.-E.
  (See online at https://doi.org/10.1128/JB.00923-09)
• (2009) In vivo mutational analysis of the Bacillus subtilis lov-domain containing protein YtvA: mechanism of lightactivation of the general stress response. J. Biol. Chem. 284 24958 – 24964
  Perez, M.A., Vreede, J., Tang, Y., Bende, O., Losi, A., Gärtner, W., and Hellingwerf, K.
  (See online at https://doi.org/10.1074/jbc.M109.033316)
• (2009) Mutual Exchange of Kinetic Properties by Extended Mutagenesis in Two Short LOV Domain Proteins from Pseudomonas putida. Biochemistry 48 10321-10333
  Jentzsch, K., Wirtz, A., Circolone, F., Drepper, T., Losi, A., Gärtner, W., Jaeger, K.-E., and Krauss, U.
  (See online at https://doi.org/10.1021/bi901115z)
• (2009) Novel Blue Light-Sensitive Proteins from a Metagenomic Approach. Env. Microbiol. 11 2388-2399
  Pathak, G.P., Ehrenreich, A., Losi, A., Streit, W., and Gärtner, W.
  (See online at https://doi.org/10.1111/j.1462-2920.2009.01967.x)
• (2010) Detection and isolation of selected genes of interest from metagenomic libraries by a DNA microarray approach. In: Methods in Molecular Biology, Streit, W.R. (ed.), pp. 299-324, Humana Press, Springer, New York
  Pathak, G.P. and Gärtner, W.
  (See online at https://doi.org/10.1007/978-1-60761-823-2_21)
• (2010) Interdomain signalling in the blue light-sensing and GTP binding protein YtvA: A mutagenesis study uncovering the importance of specific protein sites. Photobiol. Photochem. Sci. 9 47-56
  Tang, Y., Cao, Z., Livoti, E., Krauss, U., Jaeger, K.-E., Gärtner, W., and Losi, A.
  (See online at https://doi.org/10.1039/B9PP00075E)
• (2010) The switch that does not flip – the blue-light receptor YtvA from Bacillus subtilis adopts an elongated dimer conformation independent of the activation state. J. Mol. Biol. 403 78-87
  Jurk, M., Dorn, M., Kikhney, A., Svergun, D., Gärtner, W., and Schmieder, P.
  (See online at https://doi.org/10.1016/j.jmb.2010.08.036)