The senescence associated barley nuclear protein HvS40, a member of the DUF584-family - its regulation and putative function during leaf senescence
Final Report Abstract
The major goal of this project was to characterize the senescence associated gene HvS40 of barley as a key regulator of leaf senescence. It was a cooperation with the group of K. Krupinska who created transgenic barley plants with altered expression of the gene and analyzed its subcellular localization and dual coding structure. We analyzed its regulation under developmental and drought stress-induced senescence. We could show that in both cases HvS40 is induced and is under control of the upstream regulator HvWHY1. Knockdown lines of this protein delayed both, developmental and stress-induced senescence processes and also induction of HvS40. One interesting finding was that epigenetic control mechanisms are involved in this regulation. Via ChIP analyses stress-responsive loading with histone modifications of promoter and coding sequence of HvS40 could be shown. While in wild type plants the euchromatic mark H3K9Ac was increased at onset of senescence at HvS40, in plants with impaired accumulation of HvWHY1 this mark was not established. The results show that WHIRLY1 knockdown sets in motion a delay in senescence that involves all aspects of gene expression, including changes in chromatin structure.
Publications
- Identification of WHIRLY1 as a factor binding to the promoter of the stress and senescence associated gene HvS40. J Plant Growth Regul 33:91-105
Krupinska K, Dähnhardt D, Fischer-Kilbienski I, Kucharewitz W, Scharrenberg C, Trösch M, Buck F
(See online at https://doi.org/10.1007/s00344-013-9378-9) - Alterations of histone modifications at the senescence-associated gene HvS40 in barley during senescence. Plant Mol Biol 89:127-141
Ay N, Janack B, Fischer A, Reuter G, Humbeck K
(See online at https://doi.org/10.1007/s11103-015-0358-2) - Das seneszenzassoziierte Gen HvS40 der Gerste. Dissertation, Christian-Albrechts-Universität Kiel
Trösch, M.
- Knockdown of WHIRLY1 affects drought stress-induced leaf senescence and histone modifications of the senescence-associated gene HvS40. Plants, Vol. 5. 2016, Issue 3, 37.
Janack B., Sosoi P., Krupinska K., Humbeck K.
(See online at https://doi.org/10.3390/plants5030037)
