hema:harakterisierung eines neuen, zellzyklusreglierenden S. Pombe Proteins das mit dem HPV16E7 Protein interagiert und entwicklungsgeschichtlich möglicherweise konserviert ist ema:harakterisierung eines neuen, zellzyklusreglierenden S. Pombe Proteins das

• Applicant: Dr. Massimo Tommasino
• Subject Area: Basic Research in Biology and Medicine
• Term: from 1997 to 2000
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 5387769

Fundamental cellular events are highly conserved throughout evolution from yeast to humans. The major transforming protein of human papillomavirus type 16 (HPV16), E7, partially retains its biological properties in yeast Schizosaccormyces pombe (S. pombe). Recently, we have identified a novel S. pombe protein (p25), which associates in vivo and in vitro with the HPV16E7 protein. Interestingly, the p25 protein level is cell cycle regulated, being completely absent in mitosis. p25 has approximately 50% homology with a protein family of yet unknown functions which appears to be conserved in budding yeast S. cerevisiae, Caenorhabditis elegans, rodents and humans. The HPV16 E7 associates with p25 through the pRb binding site of E7, a domain essential for its transforming activity. In the first part of our research program we have characterised the biological function of some of the members of the p25 family in yeast and human cells. Our initial results indicate that members of the p25 family, despite the similarity of their amino acid sequences, may be involved in different cellular pathways in different eukaryotic cells. We have evidence that some of the p25 proteins play a role in mitochondrial function and cell cycle progression in yeast cells. We have also identified a novel human protein with a molecular weight of approximately 200 kDa (p200). The presence of the three conserved motifs of the p25 proteins in human p200, suggests that this novel protein is related to the p25 proteins. We intend to focus our future studies on the characterisation of p25 biological role(s) in cell cycle control. In particular we aim to identify human p25-related protein which associates with HPV16 E7.

• DFG Programme: Priority Programmes
• Subproject of: SPP 314: Kontrolle des Zellzyklus bei Eukaryonten