Project Details
Projekt
The metabolic characteristics of stem cells
Applicant
Dr. Michael Cross
Subject Area
Nuclear Medicine, Radiotherapy, Radiobiology
Term
from 2002 to 2005
Project identifier
Deutsche Forschungsgemeinschaft (DFG) - Project number 5377976
The potential use of human stem cells in regenerative and reparative medicine is highly promising, but holds many challenges. One of the most important of these is the establishment of culture conditions for stem cells that reproduce in vitro selfrenewal and reprogramming events which are observed only rarely in vivo. There are now indications that the rich media used as the basis of most in vitro culture systems to date may not be well-suited to the metabolism of stem cells. Based on the results of preliminary work in the applicant's laboratory, a systematic optimisation of basic culture conditions for the propagation of stem cells will be undertaken, together with analyses of the relationship between stem cell metabolism, stress responses, and differentiation potential. The aim of this work is to define growth conditions appropriate to the high efficiency in vitro amplification and directed differentiation of primary stem cells.
DFG Programme
Priority Programmes
