Tracing clonal evolution in neuroblastoma by single-cell DNA sequencing (A01)

• Subject Area: Hematology, Oncology ; Bioinformatics and Theoretical Biology ; Pediatric and Adolescent Medicine
• Term: since 2023
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 493872418

This project uses single cell sequencing to gain deep insights into the evolutionary processes of high-risk neuroblastoma. Cell nuclei are isolated from neuroblastoma samples and sequencing libraries are constructed to identify sub-clonal genomic alterations. The project aims to gain insights into clonal neuroblastoma evolution during tumor spread and relapse after chemotherapy and to reveal principles of mutational selection. The results may help develop more effective treatments after validation by other methods.

• DFG Programme: Collaborative Research Centres
• Subproject of: SFB 1588: Decoding and Targeting Mechanisms of Neuroblastoma Evolution
• Applicant Institution: shared FU Berlin and HU Berlin through:; Charité - Universitätsmedizin Berlin
• Project Heads: Professor Dr. Matthias Fischer; Professor Dr. Martin Peifer