Characterisation of radicals involved in anaerobic degradation of glycol ethers, trihydroxybenzene and acetylene

Antragsteller Professor Dr. Bernhard Schink
Fachliche Zuordnung Stoffwechselphysiologie, Biochemie und Genetik der Mikroorganismen
Förderung Förderung von 1999 bis 2007
Projektkennung Deutsche Forschungsgemeinschaft (DFG) - Projektnummer 5177536
 

Projektbeschreibung

In fermenting and sulfate-reducing bacteria, the reductive transformation of benzoyl-CoA is catalyzed by an enzyme system different from that observed in nitrate-reducing bacteria. The reaction leads to cyclohexene carboxyl-CoA, and appears to be ATP-independent. Formation of a radical carbonyl derivative is proposed for the first step which is subsequently reduced to cyclohexene carboxyl-CoA. A second project deals with the fermentative degradation of ethylene glycol ethers which is supposed to proceed via a radical mechanism analogous to the reaction catalyzed by diol dehydratases. In a third project, the molybdopterin-containing enzyme acetylene hydratase will be investigated with respect to the formation of a pterin-centered radical and its role in catalysis. Clearly, in all cases protein and substrate centered radicals will be identified by EPR/ENDOR spectroscopy employing both rapid quench and spin trap techniques.
DFG-Verfahren Schwerpunktprogramme
Teilprojekt zu SPP 1071:  Radikale in der enzymatischen Katalyse
Beteiligte Person Professor Dr. Peter M. H. Kroneck