Global-genome repair of DNA-protein crosslinks: lesion detection and pathway choice (21*)

This project utilises new methodologies and genetic tools to answer key open questions on the detection and repair of covalent DNA-protein crosslinks (DPCs). The repair of DPCs involves the proteolytic destruction of the protein adduct by the DPC-specific protease SPRTN or by proteasomal degradation. To monitor DPC repair in mammalian cells, we have devised a novel technique to identify DPCs and to track their fate, which we will employ to understand how the SUMO system detects DPCs and what determines replication-independent DPC repair pathway choice. Ultimately, we will build on these insights to investigate the nature of the endogenous substrates of DPC repair pathways.

DFG Programme Collaborative Research Centres

Subproject of SFB 1361:  Regulation of DNA Repair and Genome Stability

Applicant Institution Johannes Gutenberg-Universität Mainz

Project Head Professor Dr. Julian Stingele