Global-genome repair of DNA-protein crosslinks: lesion detection and pathway choice (21*)

Subject Area Biochemistry
Term since 2023
Project identifier Deutsche Forschungsgemeinschaft (DFG) - Project number 393547839
 

Project Description

This project utilises new methodologies and genetic tools to answer key open questions on the detection and repair of covalent DNA-protein crosslinks (DPCs). The repair of DPCs involves the proteolytic destruction of the protein adduct by the DPC-specific protease SPRTN or by proteasomal degradation. To monitor DPC repair in mammalian cells, we have devised a novel technique to identify DPCs and to track their fate, which we will employ to understand how the SUMO system detects DPCs and what determines replication-independent DPC repair pathway choice. Ultimately, we will build on these insights to investigate the nature of the endogenous substrates of DPC repair pathways.
DFG Programme Collaborative Research Centres
Subproject of SFB 1361:  Regulation of DNA Repair and Genome Stability
Applicant Institution Johannes Gutenberg-Universität Mainz
Project Head Professor Dr. Julian Stingele