300kV Cryo-TEM

• Subject Area: Basic Research in Biology and Medicine
• Term: Funded in 2023
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 506518771

This proposal aims to set-up a high-end cryo-electron microscopy (cryo-EM) facility that is equipped with instrumentation for the major current techniques of cryo-EM, namely single-particle analysis, cryo-electron tomography (cryo-ET) and electron diffraction as a university core facility. This will allow the University of Freiburg to be competitive in these seminal tech-niques of current structural and cellular biology. To realize this, a high-end 300 kV transmis-sion electron microscope with a fast-counting detector and an energy filter is necessary. For sample preparation in cryo-ET and further analysis, a cryo-focussed ion beam - scanning electron microscope in combination with a correlative light-and electron microscopy set-up is also required. Finally, as these instruments produce exceptionally large amounts of data, a dedicated server and storage arry for preprocessing and data analysis are essential.

• DFG Programme: Major Research Instrumentation
• Major Instrumentation: 300kV Kryo-Transmissionselektronenmikroskop (TEM)
• Instrumentation Group: 5100 Elektronenmikroskope (Transmission)
• Applicant Institution: Albert-Ludwigs-Universität Freiburg
• Leader: Professor Dr. David Haselbach