Live-cell Imaging Super-resolution Microscope System

• Subject Area: Basic Research in Biology and Medicine
• Term: Funded in 2021
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 458673139

Here, we request a super-resolution microscope system based on STED (stimulated emission depletion) technique, which will be mainly used for live-cell STED applications. Therefore, the system should provide STED imaging modalities to reduce the amount of energy of the depletion lasers on the sample, while preserving high optical resolution in x, y and z direction. Since STED is not applicable to plant tissue, the system should provide an alternative option to significantly increase resolution in plant samples. The system will be integrated in the multi-user environment of an imaging core facility. Therefore, the handling of the system should be user-friendly, and the system should offer standard confocal functionality next to the super-resolution options.

• DFG Programme: Major Research Instrumentation
• Major Instrumentation: Hochauflösendes Mikroskopsystem für Lebendzell-Beobachtung
• Instrumentation Group: 5090 Spezialmikroskope
• Applicant Institution: Heinrich-Heine-Universität Düsseldorf
• Leader: Professorin Dr. Stefanie Weidtkamp-Peters