Dissecting the mechanism of proton and cation conductance in viroporins using vibrational spectroscopy (B09*)

• Subject Area: Structural Biology
• Term: since 2021
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 221545957

In this project, we will use infrared spectroscopic methods to provide insight into the mechanisms of proton and cation conductance of the viroporins of the mosquito-borne Dengue 1 and the West Nile viruses. We will identify key amino acid residues that may be responsible for pH sensing or gating as well as monitor changes in structure and electrostatics of the channel interior, the latter using vibrational Stark effect probes, associated with the pore opening process. Finally, we will test the influence of viroporin inhibitors on the conductance mechanism and evaluate potential antiviral-resistance conferring mutations.

• DFG Programme: Collaborative Research Centres
• Subproject of: SFB 1078: Protonation Dynamics in Protein Function
• Applicant Institution: Freie Universität Berlin
• Project Head: Dr. Jacek Artur Kozuch