We will advance multiphoton fluorescence microscopy by developing a compact optical motion compensation module that will actively compensate tissue motion during intravital imaging, for instance due to heart beat (8 Hz), breathing (3 Hz, in mm-range) or peristaltic movement of the gut in mice. The module will be comprised of a high-speed deformable mirror and optics to facilitate extended focus optical coherence tomography (xfOCT). Three-dimensional xfOCT data will be processed in real time and, in combination with novel mathematical methods that track and predict tissue deformation, these will be used to track and image inflammatory processes with cellular resolution.
DFG Programme
Collaborative Research Centres
