Improving intravital microscopy of inflammatory cell response by active motion compensation using controlled adaptive optics (A06)

Subject Area Biophysics
Term since 2021
Project identifier Deutsche Forschungsgemeinschaft (DFG) - Project number 431460824
 

Project Description

We will advance multiphoton fluorescence microscopy by developing a compact optical motion compensation module that will actively compensate tissue motion during intravital imaging, for instance due to heart beat (8 Hz), breathing (3 Hz, in mm-range) or peristaltic movement of the gut in mice. The module will be comprised of a high-speed deformable mirror and optics to facilitate extended focus optical coherence tomography (xfOCT). Three-dimensional xfOCT data will be processed in real time and, in combination with novel mathematical methods that track and predict tissue deformation, these will be used to track and image inflammatory processes with cellular resolution.
DFG Programme Collaborative Research Centres
Subproject of SFB 1450:  Multiscale imaging of organ-specific inflammation
Applicant Institution Universität Münster
Project Heads Professor Dr. Thomas Huser; Professor Dr. Benedikt Wirth