Non-equilibrium effects on the organization of protein machineries (B07)

We use single molecule resonance energy transfer (smFRET) to probe kinetics in protein interactions in real time. With this we want to address the question how energy consumption affects protein complex formation. Using multicolor smFRET and other fluorescence based methods we will investigate ATP-driven succession of dynamic protein complexes using the Hsp90 machinery, the archaellum and the ribosomal protein machinery.

DFG Programme Collaborative Research Centres

Subproject of SFB 1381:  Dynamic organization of cellular protein machineries: From biogenesis and modular assembly to function

Applicant Institution Albert-Ludwigs-Universität Freiburg

Project Head Professor Dr. Thorsten Hugel