Structure-function analyses of mammalian Timeless in genome maintenance (10)

• Subject Area: Structural Biology
• Term: from 2019 to 2022
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 393547839

Mammalian Timeless (Tim) and the Timeless-interacting protein (Tipin) are components of the replica-tion fork protection complex. To provide mechanistic insights into their functions in genome mainte-nance, we will analyse 3D structures of Tim, Tipin and their complexes with single-stranded DNA-binding RPA or DNA, using an integrated structural biology approach that includes X-ray crystallog-raphy, single particle cryo-electron microscopy, small angle X-ray scattering and crosslinking mass spectrometry. These studies will be complemented by cell-based in vivo functional analyses in order to advance our mechanistic understanding of replication stress signalling and fork protection.

• DFG Programme: Collaborative Research Centres
• Subproject of: SFB 1361: Regulation of DNA Repair and Genome Stability
• Applicant Institution: Johannes Gutenberg-Universität Mainz
• Project Head: Professorin Dr. Eva Wolf