Establishment and Role of Chromatin Structure around Chromosome Replication Origins (A26)

• Subject Area: General Genetics and Functional Genome Biology
• Term: since 2018
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 213249687

Recent evidence suggests that chromatin remodellers and histone chaperones (chromatin factors) play important roles in chromosome replication during S phase. How chromatin factors achieve this and how this is regulated during the cell cycle is poorly understood. Our goals are to determine i) how chromatin factors contribute to establish the chromatin architecture around replication origins, ii) how this influences replication efficiency and iii) how this is regulated by the cell cycle machinery. We will use biochemical reconstitution experiments, cell biological approaches, mass spectrometry as well as structural methods to study this in detail.

• DFG Programme: Collaborative Research Centres
• Subproject of: SFB 1064: Chromatin Dynamics
• Applicant Institution: Ludwig-Maximilians-Universität München
• Project Head: Dr. Christoph Kurat