We will use molecular dynamics (MD) simulations to study conformational transitions of RNA polymerase II (Pol II) and of several members of the RNA helicase family. Specifically, we aim to derive energetically and sterically feasible pathways for DNA opening and loading into the catalytic cleft of Pol II. By means of SAXS-guided MD simulations and free energy calculations, we aim to reveal the mechanisms involved in RNA loading and RNA sliding by RNA helicases.
DFG Programme
Collaborative Research Centres
