Project Details
Projekt
Time-resolved biophotonics approach cellular signaling, cell-matrix interactions and matrix remodeling mechanisms in biofabricated constructs (B08+)
Subject Area
Medical Physics, Biomedical Technology
Term
from 2018 to 2021
Project identifier
Deutsche Forschungsgemeinschaft (DFG) - Project number 326998133
In this optical engineering and bioprocess engineering project we aim to develop new systems to study in situ maturation and regulation of cell-matrix junctions of biofabricated constructs over long time periods. To this end, (i) we will advance high resolution/depth penetrating multiphoton- and light sheet technology towards a 2-photon single plane illumination microscopy (2P-SPIM), and (ii) design and engineer precision positioned mobile mini-bioreactors. We will validate these systems for select-ed bioconstructs within the consortium regarding (iii) visualization of 3D spatial patterning of focal adhesion proteins between cell and matrix as well as matrix production during long-term maturation of constructs.
DFG Programme
CRC/Transregios
Applicant Institution
Julius-Maximilians-Universität Würzburg
Project Heads
Professor Dr. Andreas Beilhack; Professor Oliver Friedrich
