Project Details
Projekt
Bioinformatics of epigenome data and integrative analysis
Applicants
Professor Dr. Benedikt Brors; Sadaf Mughal, Ph.D.
Subject Area
Hematology, Oncology
Term
since 2017
Project identifier
Deutsche Forschungsgemeinschaft (DFG) - Project number 336840530
This subproject Z02 will continue to provide a central platform for bioinformatics analysis of data from sequencing-based assays, such as whole-genome and whole-exome sequencing, chromatin immunoprecipitation with subsequent sequencing, RNA sequencing, whole-genome bisulfite sequencing as well as array-based measurement of DNA methylation. We will continue to provide a central data coordination center for the research unit and study data on different levels of epigenetic regulation in an integrative manner. In addition, we will refine workflows to analyse data from single-cell sequencing experiments, in particular with respect to automatic assignment of cell types based on marker gene expression, to extending methods towards new assays such as scM&T-seq or ChIL-seq, and to creating a consensus map of chromatin accessibility regions. We will perform data integration to disentangle regulatory complexity of epigenetic changes in aging and AML, and employ methods to infer gene regulatory networks based on this information. Finally, we will study single-cell level telomere attrition and clonal development via calling of copy-number aberrations from single-cell RNA sequencing data. This project will closely collaborate with subprojects Z01 and A03-A09 to generate better insight into processes of aging and leukemogenesis.
DFG Programme
Research Units
