Correlative cryo-light microscope (cryo-LM)

• Subject Area: Basic Research in Biology and Medicine
• Term: Funded in 2017
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 384149399

In order to prepare specimen suited for high-resolution cryo-ET, additional instruments are required. The requested correlative cryo-light microscope (cryo-LM) shall facilitate cryo-fluorescence imaging in a correlated manner with electron microscopy on the 300 kV cryo-TEM for tomography and FIB-milling / scanning electron microscopy on the dual beam cryo-FIBSEM instrument. The main purpose of the cryo-LM is thus to guide the selection of targets for cryo-ET via fluorescent labels (e.g. genetically encoded GFP). Furthermore, the instrument will be used for correlative light/electron microscopic (CLEM) applications . The instrument shall be integrated into the Charité EM core facility to facilitate the cryo-ET workflow, but at the same time provide an interface to the Charité Advanced Medical Bioimaging Core Facility (AMBIO).

• DFG Programme: Major Research Instrumentation
• Major Instrumentation: Correlative cryo-light microscope (cryo-LM)
• Instrumentation Group: 5040 Spezielle Mikroskope (außer 500-503)
• Applicant Institution: Charité - Universitätsmedizin Berlin
• Leader: Professor Dr. Christian M. T. Spahn