Modular organisation of vesicle tethering to membranes: Analysis of structural features and regulation by protein palmitoylation (C01)

• Subject Area: Structural Biology
• Term: from 2007 to 2014
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 25065445

Vesicular transport in eukaryotic cells underlies a modular organization with defined submodular activities that are functionally conserved for each transport process, irrespective of donor and target membrane. In previous work, we have initiated a structural and biochemical characterization of the multi-subunit tethering complex TRAPP (transport protein particle) and its regulation by protein palmitoylation. It is proposed to extend these studies towards a comprehensive description of the three-dimensional structure, assembly and function of TRAPP which may serve as a paradigm for tethering complexes in vesicle transport in general.

• DFG Programme: Collaborative Research Centres
• Subproject of: SFB 740: From Molecules to Modules: Organisation and Dynamics of Functional Units in Cells
• Applicant Institution: shared FU Berlin and HU Berlin through:; Charité - Universitätsmedizin Berlin
• Co-Applicant Institution: Freie Universität Berlin; Max-Delbrück-Centrum für Molekulare Medizin (MDC)
• Project Heads: Professor Dr. Udo Heinemann; Privatdozent Dr. Michael Veit