Chromosome and protein dynamics during the insertion of membrane proteins into the cell membrane (P14)

• Subject Area: Cell Biology
• Term: from 2017 to 2021
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 269423233

We will make use of single-molecule tracking to further describe the dynamics of mRNAs, ribo-somes, signal recognition particle (SRP) and of SRP receptor FtsY in Shewanella putrefaciens as Gram-negative model organism, and in Bacillus subtilis as Gram-positive organism. We will extend our studies to YidC and YlxM proteins, who function during membrane insertion, and to cold shock proteins, playing a role in mRNA localization. Changes in chromosome dynamics will be studied using superresolution fluorescence microscopy, employing computer modelling.

• DFG Programme: CRC/Transregios
• Subproject of: TRR 174: Spatiotemporal dynamics of bacterial cells
• Applicant Institution: Philipps-Universität Marburg
• Project Head: Professor Dr. Peter Graumann