High resolution microscopy using a scattering layer

• Applicant: Professor Dr. Wolfgang Osten
• Subject Area: Measurement Systems
• Term: from 2017 to 2021
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 326167230

• Final Report Year: 2021

Final Report Abstract

We developed scatter-plate microscopy, a lensless high resolution imaging technique with variable magnification, variable NA, variable working distance and variable focal length. Combined with deconvolution algorithms the method realizes diffraction limited resolution. Unfortunately, we failed in our efforts to improve the image quality by developing a customized scatter-plate design. However we were able to improve the method and extend its applicability with other means. The principles of the method can play a significant role in developing new techniques for imaging through scattering media. Within the project, we could demonstrate that the technique is also realizable in a single-shot configuration with temporal modulated scatter-plates. This opens up the possibility to use memory effect based imaging techniques for imaging through fog, smoke or emulsions. We further developed, based on the correlation technique of the scatter-plate microscope, a new lensless single-pixel imaging tool that is able to realize three-dimensional and polychromatic imaging. Our single-pixel scatter-plate microscope also benefits from variable magnification, variable NA and variable focal length. It is further easy to implement and we see the chance to miniaturize its setup.

Publications

• “Image processing for scatterplate microscopy”. German. In: Forum Bildverarbeitung 2018. Ed. by Thomas Längle, Fernando Puente León, and Michael Heizmann. KIT Scientific Publishing, 2018. isbn: 978-3-7315-0833-5
  Stephan Ludwig, Wolfgang Osten, et al.
  
• “Scatter-plate microscope: improved image acquisition”. In: Unconventional Optical Imaging. Ed. by Corinne Fournier, Marc P. Georges, and Gabriel Popescu. Vol. 10677. International Society for Optics and Photonics. SPIE, 2018, pp. 211 – 221
  Stephan Ludwig, Wolfgang Osten, et al.
  
• “Deconvolution in Scatter-plate Microscopy”. In: Imaging and Applied Optics 2019 (COSI, IS, MATH, pcAOP). Optical Society of America, 2019, CW4A.3
  Stephan Ludwig, Wolfgang Osten, et al.
  
• “Image reconstruction and enhancement by deconvolution in scatter-plate microscopy”. In: Opt. Express 27.16 (Aug. 2019), pp. 23049–23058
  Stephan Ludwig, Wolfgang Osten, et al.
  
• “Scatter-plate microscope: lens-less diffraction-limited microscopy”. In: Laser + Photonics (Jan. 2019)
  Alok Kumar Singh, Wolfgang Osten, et al.
  
• “Ensemble cross-correlation for image retrieval from the intensity signal recorded by a single pixel”. In: Optical Measurement Systems for Industrial Inspection XII. ed. by Peter Lehmann, Wolfgang Osten, and Armando Albertazzi Gonçalves Jr. Vol. 11782. International Society for Optics and Photonics. SPIE, 2021, pp. 230 –239
  Stephan Ludwig, Wolfgang Osten, et al.
  
• “Scatter-plate microscopy with coherent illumination and moving scattering media”. In: Proc. DGaO 122. Jahrestagung (2021), A6
  Stephan Ludwig, Wolfgang Osten, et al.
  
• “Scatter-plate microscopy with spatially coherent illumination and temporal scatter modulation”. In: Opt. Express 29.3 (Feb. 2021), pp. 4530–4546
  Stephan Ludwig, Wolfgang Osten, et al.
  
• “Single-pixel scatter-plate microscopy”. In: Opt. Lett. 46.10 (May 2021), pp. 1000–1000
  Stephan Ludwig, Wolfgang Osten, et al.