Neurotransmitter release: spatio-temporal characterization of membrane fusion intermediates (A04)

Subject Area Molecular Biology and Physiology of Neurons and Glial Cells
Term since 2016
Project identifier Deutsche Forschungsgemeinschaft (DFG) - Project number 278001972
 

Project Description

Project A04 examines members of two functionally distinct classes of switch modulators of neurotransmitter release (Complexin 1/2 vs -3/4 and Doc2a vs. Doc2b) at the level of in vitro reconstitution of docking/fusion assays (Söllner) and in native synapses using synaptic transmission analysis (Rosenmund). A detailed characterization of the molecular features of the distinct isoforms by biochemical and electrophysiological methods will provide new insights into the mechanism and dynamics of release modulators - the functional properties of their domains and their distinct molecular rearrangements at different stages of the membrane fusion process.
DFG Programme CRC/Transregios
Subproject of TRR 186:  Molecular Switches: Spatio-temporal Control of Cellular Signal Transmission
Applicant Institution Freie Universität Berlin
Project Heads Professor Dr. Christian Rosenmund; Professor Dr. Thomas Söllner