Static and dynamic properties of antibody proteins in solution - the effects of crowding and charge-tuning

• Applicant: Professor Dr. Frank Schreiber
• Subject Area: Statistical Physics, Nonlinear Dynamics, Complex Systems, Soft and Fluid Matter, Biological Physics ; Experimental Condensed Matter Physics
• Term: from 2016 to 2021
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 316738961

• Final Report Year: 2021

Final Report Abstract

As a central result, the project has shown that an important contribution to the physical understanding of macromolecular crowding in living systems can be achieved by systematically varying the parameters volume fraction and polydispersity of the crowding, further the temperature, the type of tracer protein, and the salt-induced charge concentration, by employing xray and neutron scattering experiments, and by comparing the results to simulations of polydisperse hard-sphere suspensions. An important aspect of the project consisted in the nanosecond observation time scale, on which hydrodynamic aspects prevail and can be uniquely probed. Understanding these hydrodynamic interactions is a requirement for the description on all longer diffusive time scales.

Publications

• Effective interactions and colloidal stability of bovine γ-globulin in solution. J. Phys. Chem. B 121 (2017) 5759
  S. Da Vela, F. Roosen-Runge, M. W. A. Skoda, R. M. J. Jacobs, T. Seydel, H. Frielinghaus, M. Sztucki, R. Schweins, F. Zhang, and F. Schreiber
  
• Nanosecond tracer diffusion as a probe of the solution structure and molecular mobility of protein assemblies: The case of ovalbumin. J. Phys. Chem. B 122 (2018) 8343
  C. Beck, M. Grimaldo, F. Roosen-Runge, M. Braun, F. Zhang, F. Schreiber, and T. Seydel
  
• Two time scales for self and collective diffusion near the critical point in a simple patchy model for proteins with floating bonds. Soft Matter 14 (2018) 8006
  J. Bleibel, M. Habiger, M. Lütje, F. Hirschmann, F. Roosen-Runge, T. Seydel, F. Zhang, F. Schreiber, and M. Oettel
  
• Dynamics of proteins in solution. Quart. Rev. Biophys. 52 (2019) e7, 1
  M. Grimaldo, F. Roosen-Runge, F. Zhang, F. Schreiber, and T. Seydel
  
• Following protein dynamics in real-time during crystallization. Cryst. Growth Des. 19 (2019) 7036
  C. Beck, M. Grimaldo, F. Roosen-Runge, R. Maier, O. Matsarskaia, M. Braun, B. Sohmen, O. Czakkel, R. Schweins, F. Zhang, T. Seydel, and F. Schreiber
  
• Protein short-time diffusion in a naturally crowded environment. J. Phys. Chem. Lett. 10 (2019) 1709
  M. Grimaldo, H. Lopez, C. Beck, F. Roosen-Runge, M. Moulin, J. Devos, V. Laux, M. Härtlein, S. Da Vela, R. Schweins, A. Mariani, F. Zhang, J.L. Barrat, M. Oettel, V.T. Forsyth, T. Seydel, and F. Schreiber
  
• Microscopic Dynamics of Liquid-Liquid Phase Separation and Domain Coarsening in a Protein Solution Revealed by X-Ray Photon Correlated Spectroscopy. Phys.Rev.Lett. 126 (2021) 138004
  A. Girelli, H. Rahmann, N. Begam, A. Ragulskaya, M. Reiser, S. Chandran, F. Westermeier, M. Sprung, F. Zhang, C. Gutt, and F. Schreiber