Determinants, dynamics and diversity of SUMO/PML nuclear bodies in the control of hepatitis B virus infection and cccDNA (16)

• Subject Area: Virology
• Term: since 2016
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 272983813

We will examine the role of posttranslational SUMO modification of HBV proteins, particularly core and HBx, and understand the role of this modification in the viral replication cycle. We will also study the role of HBx SUMOylation in proteasomal degradation of SMC5/6 via the DDB1/CUL4 E3 ubiquitin ligase complex and the implications for epigenetic regulation and transcription of cccDNA. Since not all PML nuclear bodies (NBs) contribute to cccDNA establishment, the goal is to uncover the precise composition of cccPML NBs. Since these nuclear structures might represent the sites of cccDNA synthesis, we will use our results to devise novel therapeutic approaches.

• DFG Programme: CRC/Transregios
• Subproject of: TRR 179: Determinants and dynamics of elimination versus persistence of hepatitis virus infection
• Applicant Institution: Ruprecht-Karls-Universität Heidelberg
• Project Heads: Professor Dr. Michael Nassal, until 6/2020; Professorin Dr. Sabrina Schreiner