Komponenten des Stickstoffregulations-Netzwerkes in Fusarium fujikuroi
Zusammenfassung der Projektergebnisse
During the last 3 years we were able to shed more light into the nitrogendependent regulation of secondary metabolism in F. fujikuroi: 1. AreA and AreB are not only global regulators of growth, but play important roles in regulation of secondary metabolism. 2. Whereas AreA acts mainly as positive regulator of nitrogen-repressed gene clusters, the role of AreB is more complex. AreB acts as activator or repressor under both nitrogen-limiting and –repressing conditions. 3. AreA and AreB do not only act by binding to the promoters of target genes, but affect secondary metabolism by histone modifications at SM gene clusters. 4. Nitrate represses nitrogen-regulated gene clusters with a delay compared to glutamine due to the time-consuming conversion of nitrate via nitrite and ammonium. 5. In F. fujikuroi, nitrate but not nitrite (in contrast to Aspergillus nidulans), activates the genes of the nitrate assimilation and induces shuttling of the TF NirA into the nucleus. 6. The nitrate reductase is involved in nitrate sensing in F. fujikuroi: NirA is not translocated to the nucleus in response to nitrate in the background of the NIAD deletion mutant.
Projektbezogene Publikationen (Auswahl)
- (2008). Cross-species hybridization with Fusarium verticillioides microarrays reveals new insights in Fusarium fujikuroi nitrogen regulation and the role of AreA and NMR. Euk. Cell. 7: 1831-1846
Schönig, B., Brown, D. W., Oeser, B., and Tudzynski, B.
- (2008). The impact of the ammonium permeases MepA, MepB and MepC on nitrogen-regulated secondary metabolism in Fusarium fujikuroi. Euk. Cell. 7: 187-201
Teichert, S., Rutherford, J. C., Wottawa, M., Heitman, J. and Tudzynski, B.
- (2010). The bZIP transcription factor MeaB mediates nitrogen metabolite repression at specific loci. Euk. Cell 9: 1588-1601
Wagner, D., Schmeinck, A., Mos,M., Morozov, I.Y., Caddick, M.X., Tudzynski, B.
- (2013) A Sensing Role of the Glutamine Synthetase in the Nitrogen Regulation Network in Fusarium fujikuroi. PLoS ONE 8(11): e80740
Wagner, D., Wiemann, P., Huß, K., Brandt, U., Fleißner, A. and Tudzynski, B.
(Siehe online unter https://doi.org/10.1371/journal.pone.0080740) - (2014) Nitrogen regulation of secondary metabolism in fungi. Front Microbiol.
Tudzynski, B.
(Siehe online unter https://dx.doi.org/10.3389/fmicb.2014.00656) - (2014) The interplay between the GATA transcription factors AreA, the global nitrogen regulator and AreB in Fusarium fujikuroi. Mol Microbiol. 91: 472–493
Michielse, C.B., Pfannmüller, A., Macios, M., Rengers, P., Dzikowska, A., Tudzynski, B.
(Siehe online unter https://doi.org/10.1111/mmi.12472) - (2015) The general amino acid permease FfGap1 of Fusarium fujikuroi is sorted to the vacuole in a nitrogen-dependent, but Npr1 kinase-independent manner. PLoS One , 10(4):e0125487
Pfannmüller, A., Wagner, D., Sieber CM, Schönig, B., Boeckstaens, M., Marini, A.-M., Tudzynski, B.
- (2017) Comparative transcriptome and proteome analysis reveals a global impact of the nitrogen regulators AreA and AreB on secondary metabolism in Fusarium fujikuroi. PLoS ONE 12(4): e0176194
Pfannmüller, A., Leufken, J., Studt, L., Michielse, C.B., Sieber, C.M.K., Güldener, U., Hawat, S., Hippler, M., Fufezan, C., and Tudzynski, B.
(Siehe online unter https://doi.org/10.1371/journal.pone.0176194) - (2017) Nitrate Assimilation in Fusarium fujikuroi is controlled by multiple levels of regulation. Front Microbiol. 8:381
Pfannmüller A, Boysen J, Tudzynski B.
(Siehe online unter https://doi.org/10.3389/fmicb.2017.00381)
