Architecture and structural dynamics of core components of the translational assembly scaffold (A06)

• Subject Area: Biochemistry
• Term: from 2016 to 2023
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 189682160

The central objective of this project is to better understand the structural configuration and regulation of the 60S ribosome biogenesis machinery, focusing on the DEAD-box ATPase family members involved in 60S assembly. To this end, crosslinking coupled to mass spectrometry, integrated modeling and cryo-electron microscopy will be applied to affinity purified protein complexes from cells to help elucidate the molecular function of DEAD-box proteins in the early steps of ribosome assembly. The project also aims to shift the focus from the mere structural elucidation of ribosomal assembly intermediates to a better understanding of how 60S biogenesis is actually regulated. To this end we will use structural mass spectrometry and proteomics to obtain a better picture of the occurrence and potentially also functional relevance of specific PTMs for 60S ribosome biogenesis.

• DFG Programme: Collaborative Research Centres
• Subproject of: SFB 969: Chemical and Biological Principles of Cellular Proteostasis
• Applicant Institution: Universität Konstanz
• Project Head: Professor Dr. Florian Stengel