Autophagy in the cellular response to genotoxic stress (B02)

• Subject Area: Cell Biology ; Biochemistry
• Term: from 2016 to 2019
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 259130777

This project aims to define the molecular events that activate autophagy in response to DNA damage and to determine the scope of autophagy in the degradation and/or sequestration of nuclear proteins. To this end, we will chemically inhibit or knockdown kinases involved in DNA damage signaling and screen for activation of autophagy in a medium throughput manner using a flow cytometry-based assay, thereby identifying and subsequently characterizing critical regulators of autophagy. Further, we will analyze how autophagy affects cell fate upon DNA damage and identify DNA repair factors and chromatin-associated proteins that are targeted by autophagy after DNA damage.

• DFG Programme: Collaborative Research Centres
• Subproject of: SFB 1177: Molecular and Functional Characterization of Selective Autophagy
• Applicant Institution: Goethe-Universität Frankfurt am Main
• Co-Applicant Institution: Institut für Molekulare Biologie gGmbH
• Project Head: Professorin Dr. Petra Beli